8EW1

Structure of Bacple_01703-E145L

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Glycosyl hydrolase family 16	polymer	267	31736.6	3	UniProt (B5CYA6) Pfam (PF00722)	Phocaeicola plebeius DSM 17135 (Bacteroides plebeius)
2	D, E, F (D, E, F)	beta-D-galactopyranose-(1-4)-6-O-sulfo-alpha-L-galactopyranose-(1-3)-beta-D-galactopyranose	branched		584.5	3	In PDB
3	G (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	H, K, M (A, B, C)	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
5	I (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	J, L, N (A, B, C)	ACETATE ION	non-polymer		59.0	3	Chemie (ACT)
7	O, P, Q (A, B, C)	water	water		18.0	504	Chemie (HOH)

Sequence modifications

A, B, C: 12 - 267 (UniProt: B5CYA6)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Gly 11	-	expression tag
Leu 145	Glu 178	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	95209.8
Branched	Number of molecules	3
	Total formula weight	1753.5
Non-Polymers*	Number of molecules	8
	Total formula weight	424.9
All*	Total formula weight	97388.2

*Water molecules are not included.