8EW1

Structure of Bacple_01703-E145L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	273.15
Detector technology	PIXEL
Collection date	2019-05-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 31
Unit cell lengths	85.720, 85.720, 112.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.800 - 1.800
R-factor	0.2285
Rwork	0.227
R-free	0.25510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8ep4
RMSD bond length	0.003
RMSD bond angle	0.620
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.800	2.000
High resolution limit [Å]	1.800	1.800
Number of reflections	85616	15141
<I/σ(I)>	8.35	3.22
Completeness [%]	98.0
Redundancy	10
CC(1/2)	0.990	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	20% PEG 8K, 0.1 M Hepes pH 7.5 and 0.2 M calcium acetate