8EVB

Cryo-EM structure of cGMP bound truncated human CNGA3/CNGB3 channel in lipid nanodisc, pre-open state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Cyclic nucleotide-gated cation channel alpha-3	polymer	552	63402.8	3	UniProt (Q16281) Pfam (PF00520) Pfam (PF00027) Pfam (PF16526)	Homo sapiens (human)	Cone photoreceptor cGMP-gated channel subunit alpha,Cyclic nucleotide-gated channel alpha-3,CNG channel alpha-3,CNG-3,CNG3
2	D (D)	Cyclic nucleotide-gated cation channel beta-3	polymer	740	84637.2	1	UniProt (Q9NQW8) Pfam (PF00520) Pfam (PF00027)	Homo sapiens (human)	Cone photoreceptor cGMP-gated channel subunit beta,Cyclic nucleotide-gated cation channel modulatory subunit,Cyclic nucleotide-gated channel beta-3,CNG channel beta-3
3	E, G, I (A, B, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
4	F, H, J, K (A, B, C, D)	CYCLIC GUANOSINE MONOPHOSPHATE	non-polymer		345.2	4	Chemie (PCG)

Sequence modifications

A, B, C: 151 - 694 (UniProt: Q16281)

PDB	External Database	Details
Met 143	-	initiating methionine
Asp 144	-	expression tag
Tyr 145	-	expression tag
Lys 146	-	expression tag
Asp 147	-	expression tag
Asp 148	-	expression tag
Asp 149	-	expression tag
Asp 150	-	expression tag

D: 79 - 809 (UniProt: Q9NQW8)

PDB	External Database	Details
Met 70	-	initiating methionine
Asp 71	-	expression tag
Tyr 72	-	expression tag
Lys 73	-	expression tag
Asp 74	-	expression tag
Asp 75	-	expression tag
Asp 76	-	expression tag
Asp 77	-	expression tag
Lys 78	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	274845.7
Non-Polymers*	Number of molecules	7
	Total formula weight	2044.4
All*	Total formula weight	276890.1

*Water molecules are not included.