8ET3

Cryo-EM structure of a delivery complex containing the SspB adaptor, an ssrA-tagged substrate, and the AAA+ ClpXP protease

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	ATP-dependent Clp protease ATP-binding subunit ClpX	polymer	424	46414.8	6	UniProt (C3TLS7) Pfam (PF06689) Pfam (PF07724) Pfam (PF10431) UniProt (by SIFTS) (P0A6H1)	Escherichia coli
2	G, H, I, J, K... (H, I, J, K, L...)	ATP-dependent Clp protease proteolytic subunit	polymer	207	23468.9	7	UniProt (P0A6G7) Pfam (PF00574)	Escherichia coli	Caseinolytic protease,Endopeptidase Clp,Heat shock protein F21.5,Protease Ti
3	N (S)	Green fluorescent protein	polymer	273	30855.4	1	UniProt (P42212) Pfam (PF01353)	Escherichia coli
4	O, P (Y, Z)	Stringent starvation protein B	polymer	165	18279.4	2	UniProt (P0AFZ4) Pfam (PF04386) UniProt (by SIFTS) (P0AFZ3)	Escherichia coli
5	Q, R, S, T (A, B, C, D)	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	4	Chemie (AGS)
6	U, V (E, F)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)

Sequence modifications

A, B, C, D, E, F: 1 - 424 (UniProt: C3TLS7)

PDB	External Database	Details
Glu 408	Lys 408	conflict

H, I, J, K, L, M, N: 2 - 193 (UniProt: P0A6G7)

PDB	External Database	Details
Glu 194	-	expression tag
Asn 195	-	expression tag
Leu 196	-	expression tag
Tyr 197	-	expression tag
Phe 198	-	expression tag
Gln 199	-	expression tag
Ser 200	-	expression tag
Leu 201	-	expression tag
Glu 202	-	expression tag
His 203	-	expression tag
His 204	-	expression tag
His 205	-	expression tag
His 206	-	expression tag
His 207	-	expression tag
His 208	-	expression tag

S: 28 - 263 (UniProt: P42212)

PDB	External Database	Details
Met 2	-	initiating methionine
Gly 3	-	expression tag
Ser 4	-	expression tag
Ser 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
Asp 12	-	expression tag
Tyr 13	-	expression tag
Asp 14	-	expression tag
Ile 15	-	expression tag
Pro 16	-	expression tag
Thr 17	-	expression tag
Thr 18	-	expression tag
Glu 19	-	expression tag
Asn 20	-	expression tag
Leu 21	-	expression tag
Tyr 22	-	expression tag
Phe 23	-	expression tag
Gln 24	-	expression tag
Gly 25	-	expression tag
Ser 26	-	expression tag
Arg 27	-	expression tag
Gly 90	Ser 65	conflict
Ala 97	Ser 72	conflict
Ala 264	-	expression tag
Ala 265	-	expression tag
Asn 266	-	expression tag
Asp 267	-	expression tag
Glu 268	-	expression tag
Asn 269	-	expression tag
Tyr 270	-	expression tag
Ala 271	-	expression tag
Leu 272	-	expression tag
Ala 273	-	expression tag
Ala 274	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	510185.3
Non-Polymers*	Number of molecules	6
	Total formula weight	2947.4
All*	Total formula weight	513132.7

*Water molecules are not included.