8ET3

Cryo-EM structure of a delivery complex containing the SspB adaptor, an ssrA-tagged substrate, and the AAA+ ClpXP protease

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002020	molecular_function	protease binding
A	0004176	molecular_function	ATP-dependent peptidase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005829	cellular_component	cytosol
A	0006457	biological_process	protein folding
A	0008270	molecular_function	zinc ion binding
A	0009368	cellular_component	endopeptidase Clp complex
A	0009376	cellular_component	HslUV protease complex
A	0016887	molecular_function	ATP hydrolysis activity
A	0030164	biological_process	protein denaturation
A	0042802	molecular_function	identical protein binding
A	0043335	biological_process	protein unfolding
A	0046872	molecular_function	metal ion binding
A	0046983	molecular_function	protein dimerization activity
A	0051082	molecular_function	unfolded protein binding
A	0051301	biological_process	cell division
A	0051603	biological_process	proteolysis involved in protein catabolic process
A	0097718	molecular_function	disordered domain specific binding
A	0140662	molecular_function	ATP-dependent protein folding chaperone
B	0002020	molecular_function	protease binding
B	0004176	molecular_function	ATP-dependent peptidase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005829	cellular_component	cytosol
B	0006457	biological_process	protein folding
B	0008270	molecular_function	zinc ion binding
B	0009368	cellular_component	endopeptidase Clp complex
B	0009376	cellular_component	HslUV protease complex
B	0016887	molecular_function	ATP hydrolysis activity
B	0030164	biological_process	protein denaturation
B	0042802	molecular_function	identical protein binding
B	0043335	biological_process	protein unfolding
B	0046872	molecular_function	metal ion binding
B	0046983	molecular_function	protein dimerization activity
B	0051082	molecular_function	unfolded protein binding
B	0051301	biological_process	cell division
B	0051603	biological_process	proteolysis involved in protein catabolic process
B	0097718	molecular_function	disordered domain specific binding
B	0140662	molecular_function	ATP-dependent protein folding chaperone
C	0002020	molecular_function	protease binding
C	0004176	molecular_function	ATP-dependent peptidase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005829	cellular_component	cytosol
C	0006457	biological_process	protein folding
C	0008270	molecular_function	zinc ion binding
C	0009368	cellular_component	endopeptidase Clp complex
C	0009376	cellular_component	HslUV protease complex
C	0016887	molecular_function	ATP hydrolysis activity
C	0030164	biological_process	protein denaturation
C	0042802	molecular_function	identical protein binding
C	0043335	biological_process	protein unfolding
C	0046872	molecular_function	metal ion binding
C	0046983	molecular_function	protein dimerization activity
C	0051082	molecular_function	unfolded protein binding
C	0051301	biological_process	cell division
C	0051603	biological_process	proteolysis involved in protein catabolic process
C	0097718	molecular_function	disordered domain specific binding
C	0140662	molecular_function	ATP-dependent protein folding chaperone
D	0002020	molecular_function	protease binding
D	0004176	molecular_function	ATP-dependent peptidase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005829	cellular_component	cytosol
D	0006457	biological_process	protein folding
D	0008270	molecular_function	zinc ion binding
D	0009368	cellular_component	endopeptidase Clp complex
D	0009376	cellular_component	HslUV protease complex
D	0016887	molecular_function	ATP hydrolysis activity
D	0030164	biological_process	protein denaturation
D	0042802	molecular_function	identical protein binding
D	0043335	biological_process	protein unfolding
D	0046872	molecular_function	metal ion binding
D	0046983	molecular_function	protein dimerization activity
D	0051082	molecular_function	unfolded protein binding
D	0051301	biological_process	cell division
D	0051603	biological_process	proteolysis involved in protein catabolic process
D	0097718	molecular_function	disordered domain specific binding
D	0140662	molecular_function	ATP-dependent protein folding chaperone
E	0002020	molecular_function	protease binding
E	0004176	molecular_function	ATP-dependent peptidase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005829	cellular_component	cytosol
E	0006457	biological_process	protein folding
E	0008270	molecular_function	zinc ion binding
E	0009368	cellular_component	endopeptidase Clp complex
E	0009376	cellular_component	HslUV protease complex
E	0016887	molecular_function	ATP hydrolysis activity
E	0030164	biological_process	protein denaturation
E	0042802	molecular_function	identical protein binding
E	0043335	biological_process	protein unfolding
E	0046872	molecular_function	metal ion binding
E	0046983	molecular_function	protein dimerization activity
E	0051082	molecular_function	unfolded protein binding
E	0051301	biological_process	cell division
E	0051603	biological_process	proteolysis involved in protein catabolic process
E	0097718	molecular_function	disordered domain specific binding
E	0140662	molecular_function	ATP-dependent protein folding chaperone
F	0002020	molecular_function	protease binding
F	0004176	molecular_function	ATP-dependent peptidase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005829	cellular_component	cytosol
F	0006457	biological_process	protein folding
F	0008270	molecular_function	zinc ion binding
F	0009368	cellular_component	endopeptidase Clp complex
F	0009376	cellular_component	HslUV protease complex
F	0016887	molecular_function	ATP hydrolysis activity
F	0030164	biological_process	protein denaturation
F	0042802	molecular_function	identical protein binding
F	0043335	biological_process	protein unfolding
F	0046872	molecular_function	metal ion binding
F	0046983	molecular_function	protein dimerization activity
F	0051082	molecular_function	unfolded protein binding
F	0051301	biological_process	cell division
F	0051603	biological_process	proteolysis involved in protein catabolic process
F	0097718	molecular_function	disordered domain specific binding
F	0140662	molecular_function	ATP-dependent protein folding chaperone
H	0004176	molecular_function	ATP-dependent peptidase activity
H	0004252	molecular_function	serine-type endopeptidase activity
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006508	biological_process	proteolysis
H	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
H	0008236	molecular_function	serine-type peptidase activity
H	0009266	biological_process	response to temperature stimulus
H	0009314	biological_process	response to radiation
H	0009368	cellular_component	endopeptidase Clp complex
H	0009376	cellular_component	HslUV protease complex
H	0009408	biological_process	response to heat
H	0010498	biological_process	proteasomal protein catabolic process
H	0016020	cellular_component	membrane
H	0042802	molecular_function	identical protein binding
H	0043068	biological_process	positive regulation of programmed cell death
H	0051117	molecular_function	ATPase binding
I	0004176	molecular_function	ATP-dependent peptidase activity
I	0004252	molecular_function	serine-type endopeptidase activity
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0006508	biological_process	proteolysis
I	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
I	0008236	molecular_function	serine-type peptidase activity
I	0009266	biological_process	response to temperature stimulus
I	0009314	biological_process	response to radiation
I	0009368	cellular_component	endopeptidase Clp complex
I	0009376	cellular_component	HslUV protease complex
I	0009408	biological_process	response to heat
I	0010498	biological_process	proteasomal protein catabolic process
I	0016020	cellular_component	membrane
I	0042802	molecular_function	identical protein binding
I	0043068	biological_process	positive regulation of programmed cell death
I	0051117	molecular_function	ATPase binding
J	0004176	molecular_function	ATP-dependent peptidase activity
J	0004252	molecular_function	serine-type endopeptidase activity
J	0005515	molecular_function	protein binding
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0006508	biological_process	proteolysis
J	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
J	0008236	molecular_function	serine-type peptidase activity
J	0009266	biological_process	response to temperature stimulus
J	0009314	biological_process	response to radiation
J	0009368	cellular_component	endopeptidase Clp complex
J	0009376	cellular_component	HslUV protease complex
J	0009408	biological_process	response to heat
J	0010498	biological_process	proteasomal protein catabolic process
J	0016020	cellular_component	membrane
J	0042802	molecular_function	identical protein binding
J	0043068	biological_process	positive regulation of programmed cell death
J	0051117	molecular_function	ATPase binding
K	0004176	molecular_function	ATP-dependent peptidase activity
K	0004252	molecular_function	serine-type endopeptidase activity
K	0005515	molecular_function	protein binding
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0006508	biological_process	proteolysis
K	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
K	0008236	molecular_function	serine-type peptidase activity
K	0009266	biological_process	response to temperature stimulus
K	0009314	biological_process	response to radiation
K	0009368	cellular_component	endopeptidase Clp complex
K	0009376	cellular_component	HslUV protease complex
K	0009408	biological_process	response to heat
K	0010498	biological_process	proteasomal protein catabolic process
K	0016020	cellular_component	membrane
K	0042802	molecular_function	identical protein binding
K	0043068	biological_process	positive regulation of programmed cell death
K	0051117	molecular_function	ATPase binding
L	0004176	molecular_function	ATP-dependent peptidase activity
L	0004252	molecular_function	serine-type endopeptidase activity
L	0005515	molecular_function	protein binding
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0006508	biological_process	proteolysis
L	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
L	0008236	molecular_function	serine-type peptidase activity
L	0009266	biological_process	response to temperature stimulus
L	0009314	biological_process	response to radiation
L	0009368	cellular_component	endopeptidase Clp complex
L	0009376	cellular_component	HslUV protease complex
L	0009408	biological_process	response to heat
L	0010498	biological_process	proteasomal protein catabolic process
L	0016020	cellular_component	membrane
L	0042802	molecular_function	identical protein binding
L	0043068	biological_process	positive regulation of programmed cell death
L	0051117	molecular_function	ATPase binding
M	0004176	molecular_function	ATP-dependent peptidase activity
M	0004252	molecular_function	serine-type endopeptidase activity
M	0005515	molecular_function	protein binding
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0006508	biological_process	proteolysis
M	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
M	0008236	molecular_function	serine-type peptidase activity
M	0009266	biological_process	response to temperature stimulus
M	0009314	biological_process	response to radiation
M	0009368	cellular_component	endopeptidase Clp complex
M	0009376	cellular_component	HslUV protease complex
M	0009408	biological_process	response to heat
M	0010498	biological_process	proteasomal protein catabolic process
M	0016020	cellular_component	membrane
M	0042802	molecular_function	identical protein binding
M	0043068	biological_process	positive regulation of programmed cell death
M	0051117	molecular_function	ATPase binding
N	0004176	molecular_function	ATP-dependent peptidase activity
N	0004252	molecular_function	serine-type endopeptidase activity
N	0005515	molecular_function	protein binding
N	0005737	cellular_component	cytoplasm
N	0005829	cellular_component	cytosol
N	0006508	biological_process	proteolysis
N	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
N	0008236	molecular_function	serine-type peptidase activity
N	0009266	biological_process	response to temperature stimulus
N	0009314	biological_process	response to radiation
N	0009368	cellular_component	endopeptidase Clp complex
N	0009376	cellular_component	HslUV protease complex
N	0009408	biological_process	response to heat
N	0010498	biological_process	proteasomal protein catabolic process
N	0016020	cellular_component	membrane
N	0042802	molecular_function	identical protein binding
N	0043068	biological_process	positive regulation of programmed cell death
N	0051117	molecular_function	ATPase binding
S	0003674	molecular_function	molecular_function
S	0005575	cellular_component	cellular_component
S	0006091	biological_process	generation of precursor metabolites and energy
S	0008218	biological_process	bioluminescence
Y	0003723	molecular_function	RNA binding
Y	0005515	molecular_function	protein binding
Y	0005829	cellular_component	cytosol
Y	0005840	cellular_component	ribosome
Y	0009376	cellular_component	HslUV protease complex
Y	0032781	biological_process	positive regulation of ATP-dependent activity
Y	0042803	molecular_function	protein homodimerization activity
Y	0045732	biological_process	positive regulation of protein catabolic process
Y	0051117	molecular_function	ATPase binding
Y	0060090	molecular_function	molecular adaptor activity
Y	1903052	biological_process	positive regulation of proteolysis involved in protein catabolic process
Z	0003723	molecular_function	RNA binding
Z	0005515	molecular_function	protein binding
Z	0005829	cellular_component	cytosol
Z	0005840	cellular_component	ribosome
Z	0009376	cellular_component	HslUV protease complex
Z	0032781	biological_process	positive regulation of ATP-dependent activity
Z	0042803	molecular_function	protein homodimerization activity
Z	0045732	biological_process	positive regulation of protein catabolic process
Z	0051117	molecular_function	ATPase binding
Z	0060090	molecular_function	molecular adaptor activity
Z	1903052	biological_process	positive regulation of proteolysis involved in protein catabolic process

Functional Information from PROSITE/UniProt

site_id	PS00381
Number of Residues	12
Details	CLP_PROTEASE_SER Endopeptidase Clp serine active site. TicMGqAASMGA

Chain	Residue	Details
H	THR89-ALA100

---

site_id	PS00382
Number of Residues	14
Details	CLP_PROTEASE_HIS Endopeptidase Clp histidine active site. RfclPnsrVMIHQP

Chain	Residue	Details
H	ARG111-PRO124

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	24
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU01250, ECO:0000269|PubMed:14525985

Chain	Residue	Details
A	CYS15
C	CYS18
C	CYS37
C	CYS40
D	CYS15
D	CYS18
D	CYS37
D	CYS40
E	CYS15
E	CYS18
E	CYS37
A	CYS18
E	CYS40
F	CYS15
F	CYS18
F	CYS37
F	CYS40
A	CYS37
A	CYS40
B	CYS15
B	CYS18
B	CYS37
B	CYS40
C	CYS15

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00175, ECO:0000269|PubMed:19914167, ECO:0000269|PubMed:23622246

Chain	Residue	Details
A	PRO120
L	ASP171
M	HIS122
M	ASP171
N	HIS122
N	ASP171
B	PRO120
C	PRO120
D	PRO120
E	PRO120
F	PRO120
K	HIS122
K	ASP171
L	HIS122

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
H	GLY68	electrostatic stabiliser
H	SER97	covalent catalysis, proton shuttle (general acid/base)
H	MET98	electrostatic stabiliser
H	HIS122	proton shuttle (general acid/base)
H	ASP171	electrostatic stabiliser, modifies pKa

---

site_id	MCSA2
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
I	GLY68	electrostatic stabiliser
I	SER97	covalent catalysis, proton shuttle (general acid/base)
I	MET98	electrostatic stabiliser
I	HIS122	proton shuttle (general acid/base)
I	ASP171	electrostatic stabiliser, modifies pKa

---

site_id	MCSA3
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
J	GLY68	electrostatic stabiliser
J	SER97	covalent catalysis, proton shuttle (general acid/base)
J	MET98	electrostatic stabiliser
J	HIS122	proton shuttle (general acid/base)
J	ASP171	electrostatic stabiliser, modifies pKa

---

site_id	MCSA4
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
K	GLY68	electrostatic stabiliser
K	SER97	covalent catalysis, proton shuttle (general acid/base)
K	MET98	electrostatic stabiliser
K	HIS122	proton shuttle (general acid/base)
K	ASP171	electrostatic stabiliser, modifies pKa

---

site_id	MCSA5
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
L	GLY68	electrostatic stabiliser
L	SER97	covalent catalysis, proton shuttle (general acid/base)
L	MET98	electrostatic stabiliser
L	HIS122	proton shuttle (general acid/base)
L	ASP171	electrostatic stabiliser, modifies pKa

---

site_id	MCSA6
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
M	GLY68	electrostatic stabiliser
M	SER97	covalent catalysis, proton shuttle (general acid/base)
M	MET98	electrostatic stabiliser
M	HIS122	proton shuttle (general acid/base)
M	ASP171	electrostatic stabiliser, modifies pKa

---

site_id	MCSA7
Number of Residues	5
Details	M-CSA 912

Chain	Residue	Details
N	GLY68	electrostatic stabiliser
N	SER97	covalent catalysis, proton shuttle (general acid/base)
N	MET98	electrostatic stabiliser
N	HIS122	proton shuttle (general acid/base)
N	ASP171	electrostatic stabiliser, modifies pKa

---