8ES8
CryoEM structure of PN45545 TCR-CD3 in complex with HLA-A2 MAGEA4 (230-239)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (Z, Y) | T-cell surface glycoprotein CD3 zeta chain | polymer | 173 | 19654.5 | 2 | UniProt (P20963) Pfam (PF11628) Pfam (PF02189) | Homo sapiens (human) | T-cell receptor T3 zeta chain |
| 2 | C (D) | T-cell surface glycoprotein CD3 delta chain | polymer | 174 | 19150.7 | 1 | UniProt (P04234) Pfam (PF16680) Pfam (PF02189) | Homo sapiens (human) | T-cell receptor T3 delta chain |
| 3 | D, F (F, E) | T-cell surface glycoprotein CD3 epsilon chain | polymer | 211 | 23432.5 | 2 | UniProt (P07766) Pfam (PF16681) Pfam (PF02189) | Homo sapiens (human) | T-cell surface antigen T3/Leu-4 epsilon chain |
| 4 | E (G) | T-cell surface glycoprotein CD3 gamma chain | polymer | 185 | 20694.6 | 1 | UniProt (P09693) Pfam (PF16680) Pfam (PF02189) | Homo sapiens (human) | T-cell receptor T3 gamma chain |
| 5 | G (A) | PN45545 TCR alpha chain | polymer | 278 | 30737.5 | 1 | Homo sapiens (Human) | ||
| 6 | H (B) | PN45545 TCR beta chain | polymer | 319 | 35891.9 | 1 | Homo sapiens (Human) | ||
| 7 | I (N) | MHC class I antigen | polymer | 277 | 32082.5 | 1 | UniProt (Q861F7) Pfam (PF00129) Pfam (PF07654) | Homo sapiens (human) | |
| 8 | J (M) | Beta-2-microglobulin | polymer | 100 | 11879.4 | 1 | UniProt (P61769) Pfam (PF07654) | Homo sapiens (human) | |
| 9 | K (P) | Melanoma-associated antigen 4 | polymer | 10 | 1134.2 | 1 | UniProt (P43358) | Homo sapiens | Cancer/testis antigen 1.4,CT1.4,MAGE-4 antigen,MAGE-41 antigen,MAGE-X2 antigen |
| 10 | L (C) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 1 | In PDB GlyTouCan (G15407YE) | |||
| 11 | M, N, O, P, Q (T, U, V, Q, J) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 5 | In PDB GlyTouCan (G42666HT) | |||
| 12 | R (Y) | CHOLESTEROL HEMISUCCINATE | non-polymer | 486.7 | 1 | Chemie (Y01) | |||
| 13 | S, T, U, V, W (G, A, B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 5 | Chemie (NAG) |
Sequence modifications
Z, Y: 1 - 164 (UniProt: P20963)
D: 1 - 171 (UniProt: P04234)
F, E: 2 - 207 (UniProt: P07766)
G: 1 - 182 (UniProt: P09693)
N: 1 - 276 (UniProt: Q861F7)
M: 1 - 99 (UniProt: P61769)
| PDB | External Database | Details |
|---|---|---|
| Gly 165 | - | expression tag |
| Ser 166 | - | expression tag |
| Gly 167 | - | expression tag |
| Leu 168 | - | expression tag |
| Glu 169 | - | expression tag |
| Val 170 | - | expression tag |
| Leu 171 | - | expression tag |
| Phe 172 | - | expression tag |
| Gln 173 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 172 | - | expression tag |
| Ser 173 | - | expression tag |
| Gly 174 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | expression tag |
| Gly 1 | - | expression tag |
| Gly 208 | - | expression tag |
| Ser 209 | - | expression tag |
| Gly 210 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 183 | - | expression tag |
| Ser 184 | - | expression tag |
| Gly 185 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 237744.7 | |
| Branched | Number of molecules | 6 |
| Total formula weight | 2708.5 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1592.8 | |
| All* | Total formula weight | 242046.0 |
