8EQS

Structure of SARS-CoV-1 Orf3a in late endosome/lysosome-like environment, MSP1D1 nanodisc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ORF3a protein	polymer	330	36268.2	2	UniProt (P59632) Pfam (PF11289)	Severe acute respiratory syndrome coronavirus (SARS-CoV)	Accessory protein 3a,Protein 3a,Protein U274,Protein X1
2	C, D (C, D)	Apolipoprotein A-I	polymer	211	24704.7	2	UniProt (P02647) Pfam (PF01442)	Homo sapiens (human)	Apo-AI,ApoA-I,Apolipoprotein A1,MSP1D1 scaffold protein
3	E, F (A, B)	1,2-dioleoyl-sn-glycero-3-phosphoethanolamine	non-polymer		744.0	2	Chemie (PEE)

Sequence modifications

A, B: 1 - 274 (UniProt: P59632)

PDB	External Database	Details
Gly 275	-	expression tag
Gly 276	-	expression tag
Arg 277	-	expression tag
Gly 278	-	expression tag
Leu 279	-	expression tag
Glu 280	-	expression tag
Val 281	-	expression tag
Leu 282	-	expression tag
Phe 283	-	expression tag
Gln 284	-	expression tag
Gly 285	-	expression tag
Pro 286	-	expression tag
Gly 287	-	expression tag
Ser 288	-	expression tag
Gly 289	-	expression tag
Gln 290	-	expression tag
Leu 291	-	expression tag
Val 292	-	expression tag
Gly 293	-	expression tag
Ser 294	-	expression tag
Gly 295	-	expression tag
Gly 296	-	expression tag
Leu 297	-	expression tag
Glu 298	-	expression tag
Gly 299	-	expression tag
Gly 300	-	expression tag
Gly 301	-	expression tag
Gly 302	-	expression tag
Trp 303	-	expression tag
Ser 304	-	expression tag
His 305	-	expression tag
Pro 306	-	expression tag
Gln 307	-	expression tag
Phe 308	-	expression tag
Glu 309	-	expression tag
Lys 310	-	expression tag
Gly 311	-	expression tag
Gly 312	-	expression tag
Gly 313	-	expression tag
Ser 314	-	expression tag
Gly 315	-	expression tag
Gly 316	-	expression tag
Gly 317	-	expression tag
Ser 318	-	expression tag
Gly 319	-	expression tag
Gly 320	-	expression tag
Gly 321	-	expression tag
Ser 322	-	expression tag
Trp 323	-	expression tag
Ser 324	-	expression tag
His 325	-	expression tag
Pro 326	-	expression tag
Gln 327	-	expression tag
Phe 328	-	expression tag
Glu 329	-	expression tag
Lys 330	-	expression tag

C, D: 23 - 211 (UniProt: P02647)

PDB	External Database	Details
Gly 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
Asp 9	-	expression tag
Tyr 10	-	expression tag
Asp 11	-	expression tag
Ile 12	-	expression tag
Pro 13	-	expression tag
Thr 14	-	expression tag
Thr 15	-	expression tag
Glu 16	-	expression tag
Asn 17	-	expression tag
Leu 18	-	expression tag
Tyr 19	-	expression tag
Phe 20	-	expression tag
Gln 21	-	expression tag
Gly 22	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	121945.9
Non-Polymers*	Number of molecules	2
	Total formula weight	1488.1
All*	Total formula weight	123434.0

*Water molecules are not included.