8ENK

Crystal structure of UAP56 in complex with Tho1, the yeast homolog of human SARNP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Spliceosome RNA helicase DDX39B	polymer	390	44905.0	2	UniProt (Q13838) Pfam (PF00270) Pfam (PF00271)	Homo sapiens (human)	56 kDa U2AF65-associated protein,ATP-dependent RNA helicase p47,DEAD box protein UAP56,HLA-B-associated transcript 1 protein
2	C (E)	Protein THO1	polymer	61	6775.7	1	UniProt (P40040) Pfam (PF18592)	Saccharomyces cerevisiae (baker's yeast)
3	D, E (M, N)	RNA	polymer	15	4547.5	2		synthetic construct
4	F, I (A, B)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
5	G, J (A, B)	BERYLLIUM TRIFLUORIDE ION	non-polymer		66.0	2	Chemie (BEF)
6	H, K (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	L, M (A, B)	water	water		18.0	17	Chemie (HOH)

Sequence modifications

A, B: 44 - 428 (UniProt: Q13838)

PDB	External Database	Details
Gly 39	-	expression tag
Ala 40	-	expression tag
Met 41	-	expression tag
Gly 42	-	expression tag
Ser 43	-	expression tag

E: 123 - 178 (UniProt: P40040)

PDB	External Database	Details
Gly 118	-	expression tag
Ala 119	-	expression tag
Met 120	-	expression tag
Gly 121	-	expression tag
Ser 122	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	105680.7
Non-Polymers*	Number of molecules	6
	Total formula weight	1035.0
All*	Total formula weight	106715.7

*Water molecules are not included.