8EHI

Cryo-EM structure of his-elemental paused elongation complex with an unfolded TL (2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	non-template DNA	polymer	32	9881.4	1		Escherichia coli
2	B	template DNA	polymer	32	9706.3	1		Escherichia coli
3	R	RNA	polymer	19	6047.6	1		Escherichia coli
4	G, H	DNA-directed RNA polymerase subunit alpha	polymer	239	26459.1	2	UniProt (P0A7Z6) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (P0A7Z4) In PDB	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
5	I	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2) In PDB	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
6	J	DNA-directed RNA polymerase subunit beta'	polymer	1407	155334.7	1	UniProt (C3SIA2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) In PDB	Escherichia coli	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
7	K	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A802) Pfam (PF01192) UniProt (by SIFTS) (P0A800) In PDB	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
8	J	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
9	J	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

G, H: 1 - 234 (UniProt: P0A7Z6)

PDB	External Database	Details
Glu 235	-	expression tag
Val 236	-	expression tag
Leu 237	-	expression tag
Phe 238	-	expression tag
Gln 239	-	expression tag

J: 2 - 1407 (UniProt: C3SIA2)

PDB	External Database	Details
Val 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	394958.6
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	395113.7

*Water molecules are not included.