8EHI
Cryo-EM structure of his-elemental paused elongation complex with an unfolded TL (2)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | non-template DNA | polymer | 32 | 9881.4 | 1 | Escherichia coli | ||
2 | B | template DNA | polymer | 32 | 9706.3 | 1 | Escherichia coli | ||
3 | R | RNA | polymer | 19 | 6047.6 | 1 | Escherichia coli | ||
4 | G, H | DNA-directed RNA polymerase subunit alpha | polymer | 239 | 26459.1 | 2 | UniProt (P0A7Z6) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (P0A7Z4) In PDB | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
5 | I | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2) In PDB | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
6 | J | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155334.7 | 1 | UniProt (C3SIA2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) In PDB | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
7 | K | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A802) Pfam (PF01192) UniProt (by SIFTS) (P0A800) In PDB | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
8 | J | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
9 | J | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
G, H: 1 - 234 (UniProt: P0A7Z6)
J: 2 - 1407 (UniProt: C3SIA2)
PDB | External Database | Details |
---|---|---|
Glu 235 | - | expression tag |
Val 236 | - | expression tag |
Leu 237 | - | expression tag |
Phe 238 | - | expression tag |
Gln 239 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Val 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 394958.6 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 155.1 | |
All* | Total formula weight | 395113.7 |