8EGB
Cryo-EM structure of consensus elemental paused elongation complex with an unfolded TL
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | non-template DNA | polymer | 32 | 9793.3 | 1 | Escherichia coli | ||
| 2 | B (B) | template DNA | polymer | 32 | 9842.3 | 1 | Escherichia coli | ||
| 3 | C (R) | RNA | polymer | 17 | 5360.1 | 1 | Escherichia coli | ||
| 4 | D, E (G, H) | DNA-directed RNA polymerase subunit alpha | polymer | 239 | 26459.1 | 2 | UniProt (P0A7Z6) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 5 | F (I) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 6 | G (J) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155334.7 | 1 | UniProt (C3SIA2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 7 | H (K) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A802) Pfam (PF01192) UniProt (by SIFTS) (P0A800) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 8 | I, J (I) | CHAPSO | non-polymer | 631.9 | 2 | Chemie (1N7) | |||
| 9 | K (J) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 10 | L, M (J) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
G, H: 1 - 234 (UniProt: P0A7Z6)
J: 2 - 1407 (UniProt: C3SIA2)
| PDB | External Database | Details |
|---|---|---|
| Glu 235 | - | expression tag |
| Val 236 | - | expression tag |
| Leu 237 | - | expression tag |
| Phe 238 | - | expression tag |
| Gln 239 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Val 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 394319.1 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1418.9 | |
| All* | Total formula weight | 395738.0 |
