8EF5

Fentanyl-bound mu-opioid receptor-Gi complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F (R, M)	Mu-type opioid receptor	polymer	367	41072.2	2	UniProt (P35372) Pfam (PF00001)	Homo sapiens (human)	M-OR-1,MOR-1,Mu opiate receptor,Mu opioid receptor,MOP,hMOP
2	B, G (A, F)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40445.1	2	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	353	39020.7	1	UniProt (P54311) Pfam (PF00400)	Rattus norvegicus (Rat)	Transducin beta chain 1
4	D (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7563.8	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (cattle)	G gamma-I
5	E (E)	scFv16	polymer	248	26408.5	1		synthetic construct
6	H, N (R, M)	N-phenyl-N-[1-(2-phenylethyl)piperidin-4-yl]propanamide	non-polymer		336.5	2	Chemie (7V7)
7	I, J, K, L, M... (R, M)	CHOLESTEROL	non-polymer		386.7	10	Chemie (CLR)

Sequence modifications

A, F: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Ala 203	Gly 203	conflict
Ser 326	Ala 326	conflict

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	236027.4
Non-Polymers*	Number of molecules	12
	Total formula weight	4539.5
All*	Total formula weight	240566.9

*Water molecules are not included.