8ECD

E. coli L-asparaginase II mutant (V27T) in complex with L-Asp

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	L-asparaginase 2	polymer	334	35703.9	4	UniProt (P00805) Pfam (PF00710) Pfam (PF17763)	Escherichia coli K-12	L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II
2	E, I, M, W (A, B, C, D)	ASPARTIC ACID	non-polymer		133.1	4	Chemie (ASP)
3	AA, CA, F, J, K... (D, A, B, C)	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
4	BA, G, H, L, O... (D, A, B, C)	1,2-ETHANEDIOL	non-polymer		62.1	12	Chemie (EDO)
5	U, V (C, D)	CITRIC ACID	non-polymer		192.1	2	Chemie (CIT)
6	DA, EA, FA, GA (A, B, C, D)	water	water		18.0	1589	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 326 (UniProt: P00805)

PDB	External Database	Details
Met -7	-	initiating methionine
Asp -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Thr 27	Val 49	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	142815.8
Non-Polymers*	Number of molecules	25
	Total formula weight	2306.1
All*	Total formula weight	145121.9

*Water molecules are not included.