8ECD
E. coli L-asparaginase II mutant (V27T) in complex with L-Asp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.559, 62.781, 141.052 |
| Unit cell angles | 90.00, 117.62, 90.00 |
Refinement procedure
| Resolution | 39.220 - 1.620 |
| R-factor | 0.1274 |
| Rwork | 0.127 |
| R-free | 0.16430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eca |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.087 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.076 | 0.562 |
| Rmeas | 0.086 | 0.659 |
| Rpim | 0.040 | 0.334 |
| Number of reflections | 145830 | 6727 |
| <I/σ(I)> | 18.12 | 2.04 |
| Completeness [%] | 97.9 | 90.9 |
| Redundancy | 4.4 | 3.4 |
| CC(1/2) | 0.994 | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2 M Ammonium citrate tribasic pH 7.0, 19% w/v Polyethylene glycol 3,350 |
