8E9X
CryoEM structure of miniGo-coupled hM4Di in complex with DCZ
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Muscarinic acetylcholine receptor M4 | polymer | 348 | 39225.3 | 1 | UniProt (P08173) Pfam (PF00001) | Homo sapiens (human) | |
| 2 | B (B) | miniGo | polymer | 225 | 25159.8 | 1 | UniProt (B3KP89) UniProt (P09471) Pfam (PF00503) | Homo sapiens (human) | |
| 3 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 339 | 37285.7 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 4 | D (D) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 5 | E (E) | scFv16 | polymer | 251 | 26679.7 | 1 | Mus musculus | ||
| 6 | F (A) | 11-(4-methylpiperazin-1-yl)-5H-dibenzo[b,e][1,4]diazepine | non-polymer | 292.4 | 1 | Chemie (WEC) |
Sequence modifications
A: 1 - 374 (UniProt: P08173)
A: 375 - 479 (UniProt: P08173)
B: 1 - 62 (PDB: 8E9X)
B: 63 - 112 (UniProt: B3KP89)
B: 113 - 225 (UniProt: P09471)
| PDB | External Database | Details |
|---|---|---|
| Cys 113 | Tyr 113 | engineered mutation |
| Gly 203 | Ala 203 | engineered mutation |
B: 1 - 62 (PDB: 8E9X)
B: 63 - 112 (UniProt: B3KP89)
| PDB | External Database | Details |
|---|---|---|
| Asp 108 | Ala 227 | conflict |
| Asp 111 | Gly 230 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 203 | Ile 332 | conflict |
| Ile 206 | Val 335 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 136211.7 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 292.4 | |
| All* | Total formula weight | 136504.0 |
