8E9X

CryoEM structure of miniGo-coupled hM4Di in complex with DCZ

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Muscarinic acetylcholine receptor M4	polymer	348	39225.3	1	UniProt (P08173) Pfam (PF00001) In PDB	Homo sapiens (human)
2	B	miniGo	polymer	225	25159.8	1	UniProt (B3KP89) UniProt (P09471) Pfam (PF00503) In PDB	Homo sapiens (human)
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	339	37285.7	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
4	D	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
5	E	scFv16	polymer	251	26679.7	1		Mus musculus
6	A	11-(4-methylpiperazin-1-yl)-5H-dibenzo[b,e][1,4]diazepine	non-polymer		292.4	1	Chemie (WEC)

Sequence modifications

A: 1 - 374 (UniProt: P08173)

PDB	External Database	Details
Cys 113	Tyr 113	engineered mutation
Gly 203	Ala 203	engineered mutation

A: 375 - 479 (UniProt: P08173)
B: 1 - 62 (PDB: 8E9X)
B: 63 - 112 (UniProt: B3KP89)

PDB	External Database	Details
Asp 108	Ala 227	conflict
Asp 111	Gly 230	conflict

B: 113 - 225 (UniProt: P09471)

PDB	External Database	Details
Ala 203	Ile 332	conflict
Ile 206	Val 335	conflict

Sequence viewer

Contents of the asymmetric unit

*Water molecules are not included.