8E91

Cryo-EM structure of substrate-free ClpX.ClpP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	ATP-dependent Clp protease ATP-binding subunit ClpX	polymer	424	46414.8	6	UniProt (C3TLS7) Pfam (PF06689) Pfam (PF07724) Pfam (PF10431) UniProt (by SIFTS) (P0A6H1) In PDB	Escherichia coli
2	H, I, J, K, L...	ATP-dependent Clp protease proteolytic subunit	polymer	207	23468.9	14	UniProt (P0A6G7) Pfam (PF00574) In PDB	Escherichia coli	Caseinolytic protease,Endopeptidase Clp,Heat shock protein F21.5,Protease Ti
3	A, B, C, D	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	4	Chemie (AGS)
4	D, B, C	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
5	E, F	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)

Sequence modifications

A, B, C, D, E, F: 1 - 424 (UniProt: C3TLS7)

PDB	External Database	Details
Glu 408	Lys 408	engineered mutation

H, I, J, K, L, M, N, h, i, j, k, l, m, n: 2 - 193 (UniProt: P0A6G7)

PDB	External Database	Details
Glu 194	-	expression tag
Asn 195	-	expression tag
Leu 196	-	expression tag
Tyr 197	-	expression tag
Phe 198	-	expression tag
Gln 199	-	expression tag
Ser 200	-	expression tag
Leu 201	-	expression tag
Glu 202	-	expression tag
His 203	-	expression tag
His 204	-	expression tag
His 205	-	expression tag
His 206	-	expression tag
His 207	-	expression tag
His 208	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	20
	Total formula weight	607053.3
Non-Polymers*	Number of molecules	9
	Total formula weight	3020.3
All*	Total formula weight	610073.6

*Water molecules are not included.