8E6N

X-ray structure of the Deinococcus radiodurans Nramp/MntH divalent transition metal transporter G223W mutant in an outward-open, manganese-bound state

Replaces:  6BU5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Divalent metal cation transporter MntH	polymer	411	44480.7	1	UniProt (Q9RTP8) Pfam (PF01566)	Deinococcus radiodurans
2	B, C (A)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
3	D, E, F, G, H... (A)	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	18	Chemie (OLC)
4	V, W, X, Y (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	4	Chemie (PEG)
5	AA, Z (A)	SPERMIDINE	non-polymer		145.2	2	Chemie (SPD)
6	BA (A)	water	water		18.0	54	Chemie (HOH)

Sequence modifications

A: 35 - 436 (UniProt: Q9RTP8)

PDB	External Database	Details
Met 26	-	expression tag
His 27	-	expression tag
His 28	-	expression tag
His 29	-	expression tag
His 30	-	expression tag
His 31	-	expression tag
His 32	-	expression tag
His 33	-	expression tag
His 34	-	expression tag
Trp 223	Gly 223	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	44480.7
Non-Polymers*	Number of molecules	26
	Total formula weight	7242.6
All*	Total formula weight	51723.2

*Water molecules are not included.