8E5K

Escherichia coli Rho-dependent transcription pre-termination complex containing 21 nt long RNA spacer, Mg-ADP-BeF3, and NusG; TEC part

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	5	NT DNA	polymer	60	18377.8	1		synthetic construct
2	6	T DNA	polymer	60	18446.8	1		synthetic construct
3	7	RNA with 21 nt long spacer	polymer	38	11993.8	1		synthetic construct
4	A	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2) In PDB	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
5	B	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	1	UniProt (P0A8T7) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04997) In PDB	Escherichia coli	RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta'
6	C, D	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
7	E	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A802) Pfam (PF01192) UniProt (by SIFTS) (P0A800) In PDB	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
8	F	Transcription termination/antitermination protein NusG	polymer	181	20560.5	1	UniProt (U9XYQ6) Pfam (PF02357) Pfam (PF00467) UniProt (by SIFTS) (P0AFG0) In PDB	Escherichia coli
9	B	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
10	B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	458933.5
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	459088.6

*Water molecules are not included.