8E4Y

Cryo-EM structure of human glycerol-3-phosphate acyltransferase 1 (GPAT1) in complex with 2-oxohexadecyl-CoA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Glycerol-3-phosphate acyltransferase 1, mitochondrial	polymer	767	86919.5	1	UniProt (Q9HCL2) Pfam (PF01553) Pfam (PF19277) In PDB	Homo sapiens (human)	GPAT-1
2	A	[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)oxolan-2-yl]methyl (3R)-3-hydroxy-2,2-dimethyl-4-oxo-4-{[3-oxo-3-({2-[(2-oxohexadecyl)sulfanyl]ethyl}amino)propyl]amino}butyl dihydrogen diphosphate (non-preferred name)	non-polymer		1005.9	1	Chemie (UKL)
3	A	(2R)-2-hydroxy-3-(phosphonooxy)propyl hexadecanoate	non-polymer		410.5	1	Chemie (NKO)

Sequence modifications

A: 80 - 828 (UniProt: Q9HCL2)

PDB	External Database	Details
Met 62	-	initiating methionine
Asp 63	-	expression tag
Tyr 64	-	expression tag
Lys 65	-	expression tag
Asp 66	-	expression tag
Asp 67	-	expression tag
Asp 68	-	expression tag
Asp 69	-	expression tag
Lys 70	-	expression tag
Gly 71	-	expression tag
Ser 72	-	expression tag
Glu 73	-	expression tag
Asn 74	-	expression tag
Leu 75	-	expression tag
Tyr 76	-	expression tag
Phe 77	-	expression tag
Gln 78	-	expression tag
Ser 79	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	86919.5
Non-Polymers*	Number of molecules	2
	Total formula weight	1416.4
All*	Total formula weight	88335.9

*Water molecules are not included.