8E3Y

Cryo-EM structure of the VPAC1R-PACAP27-Gs complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45699.4	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38534.1	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	N	Nanobody 35	polymer	138	15140.7	1		Lama glama
5	P	Pituitary adenylate cyclase-activating polypeptide	polymer	27	3154.6	1	UniProt (P18509) Pfam (PF00123) In PDB	Homo sapiens	PACAP
6	R	Vasoactive intestinal polypeptide receptor 1	polymer	462	52620.7	1	UniProt (P32241) Pfam (PF02793) Pfam (PF00002) In PDB	Homo sapiens (human)	VIP-R-1,Pituitary adenylate cyclase-activating polypeptide type II receptor,PACAP type II receptor,PACAP-R-2,PACAP-R2,VPAC1
7		water	water		18.0	14	Chemie (HOH)

Sequence modifications

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	conflict
Ala 226	Gly 226	conflict
Ala 268	Glu 268	conflict
Lys 271	Asn 271	conflict
Asp 274	Lys 274	conflict
Lys 280	Arg 280	conflict
Asp 284	Thr 284	conflict
Thr 285	Ile 285	conflict
Ser 366	Ala 366	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 28 - 457 (UniProt: P32241)

PDB	External Database	Details
Asp 15	-	expression tag
Tyr 16	-	expression tag
Lys 17	-	expression tag
Asp 18	-	expression tag
Asp 19	-	expression tag
Asp 20	-	expression tag
Asp 21	-	expression tag
Leu 22	-	expression tag
Glu 23	-	expression tag
Val 24	-	expression tag
Leu 25	-	expression tag
Phe 26	-	expression tag
Gln 27	-	expression tag
Pro 29	Gln 29	conflict
Pro 458	-	expression tag
Ala 459	-	expression tag
Gly 460	-	expression tag
Leu 461	-	expression tag
Glu 462	-	expression tag
Val 463	-	expression tag
Leu 464	-	expression tag
Phe 465	-	expression tag
Gln 466	-	expression tag
Gly 467	-	expression tag
Pro 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag
His 472	-	expression tag
His 473	-	expression tag
His 474	-	expression tag
His 475	-	expression tag
His 476	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	163010.7
All*	Total formula weight	163010.7

*Water molecules are not included.