8E3Y
Cryo-EM structure of the VPAC1R-PACAP27-Gs complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45699.4 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 350 | 38534.1 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (N) | Nanobody 35 | polymer | 138 | 15140.7 | 1 | Lama glama | ||
| 5 | E (P) | Pituitary adenylate cyclase-activating polypeptide | polymer | 27 | 3154.6 | 1 | UniProt (P18509) Pfam (PF00123) | Homo sapiens | PACAP |
| 6 | F (R) | Vasoactive intestinal polypeptide receptor 1 | polymer | 462 | 52620.7 | 1 | UniProt (P32241) Pfam (PF02793) Pfam (PF00002) | Homo sapiens (human) | VIP-R-1,Pituitary adenylate cyclase-activating polypeptide type II receptor,PACAP type II receptor,PACAP-R-2,PACAP-R2,VPAC1 |
| 7 | G, H (P, R) | water | water | 18.0 | 14 | Chemie (HOH) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
R: 28 - 457 (UniProt: P32241)
| PDB | External Database | Details |
|---|---|---|
| Asn 54 | Ser 54 | conflict |
| Ala 226 | Gly 226 | conflict |
| Ala 268 | Glu 268 | conflict |
| Lys 271 | Asn 271 | conflict |
| Asp 274 | Lys 274 | conflict |
| Lys 280 | Arg 280 | conflict |
| Asp 284 | Thr 284 | conflict |
| Thr 285 | Ile 285 | conflict |
| Ser 366 | Ala 366 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asp 15 | - | expression tag |
| Tyr 16 | - | expression tag |
| Lys 17 | - | expression tag |
| Asp 18 | - | expression tag |
| Asp 19 | - | expression tag |
| Asp 20 | - | expression tag |
| Asp 21 | - | expression tag |
| Leu 22 | - | expression tag |
| Glu 23 | - | expression tag |
| Val 24 | - | expression tag |
| Leu 25 | - | expression tag |
| Phe 26 | - | expression tag |
| Gln 27 | - | expression tag |
| Pro 29 | Gln 29 | conflict |
| Pro 458 | - | expression tag |
| Ala 459 | - | expression tag |
| Gly 460 | - | expression tag |
| Leu 461 | - | expression tag |
| Glu 462 | - | expression tag |
| Val 463 | - | expression tag |
| Leu 464 | - | expression tag |
| Phe 465 | - | expression tag |
| Gln 466 | - | expression tag |
| Gly 467 | - | expression tag |
| Pro 468 | - | expression tag |
| His 469 | - | expression tag |
| His 470 | - | expression tag |
| His 471 | - | expression tag |
| His 472 | - | expression tag |
| His 473 | - | expression tag |
| His 474 | - | expression tag |
| His 475 | - | expression tag |
| His 476 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 163010.7 | |
| All* | Total formula weight | 163010.7 |
