8E15

A computationally stabilized hMPV F protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	F	F2 protein	polymer	102	11687.2	1	UniProt (Q8B9P0) In PDB	Human metapneumovirus
2	G	F1 protein with Fibritin peptide	polymer	422	45999.2	1	UniProt (Q8B9P0) UniProt (Q76VI8) Pfam (PF00523) In PDB	Human metapneumovirus
3	A	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
4	F, G	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
5		water	water		18.0	11	Chemie (HOH)

Sequence modifications

F: 1 - 102 (UniProt: Q8B9P0)

PDB	External Database	Details
Asn 90	Ala 90	engineered mutation
Arg 100	Gln 100	engineered mutation
Arg 101	Ser 101	engineered mutation

G: 103 - 490 (UniProt: Q8B9P0)

PDB	External Database	Details
Glu 114	Thr 114	engineered mutation
Asp 130	Leu 130	engineered mutation
Leu 159	Ala 159	engineered mutation
Pro 185	Ala 185	engineered mutation
Ile 203	Val 203	engineered mutation
Gln 430	Val 430	engineered mutation
Asp 449	Val 449	engineered mutation
Ser 491	-	linker

G: 492 - 518 (UniProt: Q76VI8)

PDB	External Database	Details
His 519	-	expression tag
His 520	-	expression tag
His 521	-	expression tag
His 522	-	expression tag
His 523	-	expression tag
His 524	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	57686.4
Branched	Number of molecules	1
	Total formula weight	586.5
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	58715.4

*Water molecules are not included.