8E15

A computationally stabilized hMPV F protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	I 21 3
Unit cell lengths	178.191, 178.191, 178.191
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.620 - 2.410
R-factor	0.2058
Rwork	0.204
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wb0
RMSD bond length	0.009
RMSD bond angle	1.017
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.620	2.496
High resolution limit [Å]	2.410	2.410
Number of reflections	36363	3606
<I/σ(I)>	9.18	0.78
Completeness [%]	99.9	99.86
Redundancy	2	2
CC(1/2)	0.999	0.593

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Sodium acetate trihydrate pH 4.6, 2.0 M Sodium formate