8DP2

Crystal Structure of UDP-N-acetylmuramoylalanine--D-glutamate ligase (MurD) from Pseudomonas aeruginosa PAO1 in complex with UMA (Uridine-5'-diphosphate-N-acetylmuramoyl-L-Alanine)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	UDP-N-acetylmuramoylalanine--D-glutamate ligase	polymer	448	48348.3	1	UniProt (Q9HVZ9) Pfam (PF21799) Pfam (PF08245) Pfam (PF02875) In PDB	Pseudomonas aeruginosa PAO1	D-glutamic acid-adding enzyme,UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
2	A	URIDINE-5'-DIPHOSPHATE-N-ACETYLMURAMOYL-L-ALANINE	non-polymer		750.5	1	Chemie (UMA)
3	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	A	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
6	A	3,6,9,12,15,18,21-HEPTAOXATRICOSANE-1,23-DIOL	non-polymer		370.4	1	Chemie (PE8)
7	A	SUCCINIC ACID	non-polymer		118.1	1	Chemie (SIN)
8	A	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
9		water	water		18.0	516	Chemie (HOH)

Sequence modifications

A: 9 - 448 (UniProt: Q9HVZ9)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
Met 8	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	48348.3
Non-Polymers*	Number of molecules	8
	Total formula weight	1601.5
All*	Total formula weight	49949.8

*Water molecules are not included.