8DP2
Crystal Structure of UDP-N-acetylmuramoylalanine--D-glutamate ligase (MurD) from Pseudomonas aeruginosa PAO1 in complex with UMA (Uridine-5'-diphosphate-N-acetylmuramoyl-L-Alanine)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | UDP-N-acetylmuramoylalanine--D-glutamate ligase | polymer | 448 | 48348.3 | 1 | UniProt (Q9HVZ9) Pfam (PF21799) Pfam (PF08245) Pfam (PF02875) | Pseudomonas aeruginosa PAO1 | D-glutamic acid-adding enzyme,UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase |
| 2 | B (A) | URIDINE-5'-DIPHOSPHATE-N-ACETYLMURAMOYL-L-ALANINE | non-polymer | 750.5 | 1 | Chemie (UMA) | |||
| 3 | C (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 4 | D, E (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 5 | F (A) | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
| 6 | G (A) | 3,6,9,12,15,18,21-HEPTAOXATRICOSANE-1,23-DIOL | non-polymer | 370.4 | 1 | Chemie (PE8) | |||
| 7 | H (A) | SUCCINIC ACID | non-polymer | 118.1 | 1 | Chemie (SIN) | |||
| 8 | I (A) | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
| 9 | J (A) | water | water | 18.0 | 516 | Chemie (HOH) |
Sequence modifications
A: 9 - 448 (UniProt: Q9HVZ9)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Ala 2 | - | expression tag |
| His 3 | - | expression tag |
| His 4 | - | expression tag |
| His 5 | - | expression tag |
| His 6 | - | expression tag |
| His 7 | - | expression tag |
| Met 8 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 48348.3 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1601.5 | |
| All* | Total formula weight | 49949.8 |
