8DOM

Structure of the N358Y single variant ofserine hydroxymethyltransferase 8 from Glycine max cultivar Essex complexed with PLP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Serine hydroxymethyltransferase	polymer	492	54418.5	2	UniProt (A0A0R0IK90) Pfam (PF00464)	Glycine max (soybean)
2	C (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
3	D, E (A, B)	water	water		18.0	376	Chemie (HOH)

Sequence modifications

A, B: 1 - 471 (UniProt: A0A0R0IK90)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Tyr 358	Asn 428	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	108837.1
Non-Polymers*	Number of molecules	1
	Total formula weight	62.1
All*	Total formula weight	108899.2

*Water molecules are not included.