8DOM

Structure of the N358Y single variant ofserine hydroxymethyltransferase 8 from Glycine max cultivar Essex complexed with PLP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979180
Spacegroup name	P 2 21 21
Unit cell lengths	55.967, 128.599, 128.629
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.310 - 1.890
R-factor	0.1822
Rwork	0.180
R-free	0.21770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6uxh
RMSD bond length	0.008
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	128.630	1.930
High resolution limit [Å]	1.890	1.890
Rmerge	0.069	4.323
Rmeas	0.075
Rpim	0.027	1.678
Number of reflections	75185	33413
<I/σ(I)>	17	0.5
Completeness [%]	99.9	99.9
Redundancy	7.4	7.6
CC(1/2)	0.999	0.339

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.18 - 0.25 TMAO 0.1 M Tris (ph 8.5) 21-23 (w/v) % PEG MME 2000