8DMB

Structure of Desulfovirgula thermocuniculi IsrB (DtIsrB) in complex with omega RNA and target DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	P	Ubiquitin-like protein SMT3,IsrB protein,monomeric superfolder Green Fluorescent Protein	polymer	741	84080.9	1	UniProt (Q12306) Pfam (PF00240) In PDB	Saccharomyces cerevisiae S288C
2	W	omega RNA	polymer	284	91748.3	1		Desulfovirgula thermocuniculi
3	X	target DNA	polymer	31	9593.2	1		synthetic construct
4	Y	non-target DNA	polymer	10	3075.0	1		synthetic construct
5	W	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

P: -98 - -1 (UniProt: Q12306)

PDB	External Database	Details
Met -149	-	initiating methionine
Gly -148	-	expression tag
Ser -147	-	expression tag
Ser -146	-	expression tag
His -145	-	expression tag
His -144	-	expression tag
His -143	-	expression tag
His -142	-	expression tag
His -141	-	expression tag
His -140	-	expression tag
Ser -139	-	expression tag
Ser -138	-	expression tag
Gly -137	-	expression tag
Leu -136	-	expression tag
Val -135	-	expression tag
Pro -134	-	expression tag
Arg -133	-	expression tag
Gly -132	-	expression tag
Ser -131	-	expression tag
His -130	-	expression tag
Met -129	-	expression tag
Ala -128	-	expression tag
Ser -127	-	expression tag
Trp -126	-	expression tag
Ser -125	-	expression tag
His -124	-	expression tag
Pro -123	-	expression tag
Gln -122	-	expression tag
Phe -121	-	expression tag
Glu -120	-	expression tag
Lys -119	-	expression tag
Gly -118	-	expression tag
Gly -117	-	expression tag
Gly -116	-	expression tag
Ser -115	-	expression tag
Gly -114	-	expression tag
Gly -113	-	expression tag
Gly -112	-	expression tag
Ser -111	-	expression tag
Gly -110	-	expression tag
Gly -109	-	expression tag
Ser -108	-	expression tag
Ala -107	-	expression tag
Trp -106	-	expression tag
Ser -105	-	expression tag
His -104	-	expression tag
Pro -103	-	expression tag
Gln -102	-	expression tag
Phe -101	-	expression tag
Glu -100	-	expression tag
Lys -99	-	expression tag
Ser 0	-	linker

P: 1 - 353 (PDB: 8DMB)
P: 354 - 591 (PDB: 8DMB)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	188497.4
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	188546.0

*Water molecules are not included.