8DL2

BoGH13ASus from Bacteroides ovatus bound to acarbose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Alpha amylase, catalytic domain protein	polymer	738	84055.6	4	UniProt (A7M087)	Bacteroides ovatus ATCC 8483
2	E, H (H, K)	4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		645.6	2
3	F, I, J, N, O... (I, L, O, N, V...)	4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		645.6	6	BIRD (PRD_900007)
4	G (J)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		969.9	1	BIRD (PRD_900007)
5	K (P)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1
6	L (R)	4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		483.5	1
7	M, Q (M, S)	alpha-D-glucopyranose-(1-4)-4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		807.7	2	BIRD (PRD_900007)
8	R, Y (A, B)	alpha-D-glucopyranose	non-polymer		180.2	2	Chemie (GLC)
9	HA, MA, S, Z (C, D, A, B)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
10	EA, T (B, A)	TRIETHYLENE GLYCOL	non-polymer		150.2	2	Chemie (PGE)
11	AA, CA, DA, IA, JA... (B, C, A)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
12	PA, V (D, A)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
13	FA, KA, W (B, C, A)	ACETATE ION	non-polymer		59.0	3	Chemie (ACT)
14	GA, LA, QA, X (B, C, D, A)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
15	BA, OA (B, D)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
16	NA (D)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
17	RA, SA, TA, UA (A, B, C, D)	water	water		18.0	2276	Chemie (HOH)

Sequence modifications

A, B, C, D: 22 - 758 (UniProt: A7M087)

PDB	External Database	Details
Gly 21	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	336222.5
Branched	Number of molecules	13
	Total formula weight	8576.0
Non-Polymers*	Number of molecules	26
	Total formula weight	2142.2
All*	Total formula weight	346940.8

*Water molecules are not included.