8DH4

T7 RNA polymerase elongation complex with unnatural base dPa-DsTP pair

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I, M (B, E, I, M)	T7 RNA polymerase	polymer	883	98984.2	4	UniProt (P00573) Pfam (PF14700) Pfam (PF00940)	Escherichia phage T7	DNA-directed RNA polymerase
2	B, F, J, N (A, F, J, N)	Template strand DNA	polymer	18	5510.6	4		synthetic construct
3	C, G, K, O (C, G, K, O)	RNA	polymer	12	3851.4	4		synthetic construct
4	D, H, L, P (D, H, L, P)	Non-template strand DNA	polymer	9	2666.8	4		synthetic construct
5	Q, S (B, E)	(7P)-3-{5-O-[(R)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl]-beta-D-ribofuranosyl}-7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridine	non-polymer		573.3	2	Chemie (S96)
6	R, T (B, E)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	444051.7
Non-Polymers*	Number of molecules	4
	Total formula weight	1195.2
All*	Total formula weight	445246.9

*Water molecules are not included.