8DH4
T7 RNA polymerase elongation complex with unnatural base dPa-DsTP pair
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-12-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 78.779, 86.230, 201.463 |
Unit cell angles | 89.85, 85.39, 69.59 |
Refinement procedure
Resolution | 43.200 - 2.800 |
R-factor | 0.2334 |
Rwork | 0.233 |
R-free | 0.27450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8dh0 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHENIX (1.19) |
Refinement software | PHENIX (1.19) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.840 | 48.840 | 2.800 |
High resolution limit [Å] | 2.750 | 15.060 | 2.750 |
Rmerge | 0.099 | 0.037 | 2.239 |
Rmeas | 0.115 | 0.044 | 2.595 |
Rpim | 0.059 | 0.024 | 1.310 |
Total number of observations | 481832 | 2503 | 24587 |
Number of reflections | 125883 | 668 | 6284 |
<I/σ(I)> | 8.2 | 29 | 0.6 |
Completeness [%] | 97.8 | 85.4 | 97.8 |
Redundancy | 3.8 | 3.7 | 3.9 |
CC(1/2) | 0.995 | 0.973 | 0.253 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 298 | 10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1 |