8DH1
T7 RNA polymerase elongation complex with unnatural base dDs-PaTP pair
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E, I, L (B, E, I, L) | T7 RNA polymerase | polymer | 883 | 98984.2 | 4 | UniProt (P00573) Pfam (PF14700) Pfam (PF00940) | Escherichia phage T7 | DNA-directed RNA polymerase |
| 2 | B, F, J, M (A, F, J, M) | Template strand DNA | polymer | 18 | 5616.7 | 4 | synthetic construct | ||
| 3 | C, G, K, N (C, G, K, N) | RNA | polymer | 12 | 3851.4 | 4 | synthetic construct | ||
| 4 | D, H, O (D, H, O) | Non-template strand DNA | polymer | 9 | 2666.8 | 3 | synthetic construct | ||
| 5 | P, S, U (B, C, I) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 6 | Q (B) | 1-{5-O-[(S)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl]-beta-D-ribofuranosyl}-1H-pyrrole-2-carbaldehyde | non-polymer | 467.2 | 1 | Chemie (S8L) | |||
| 7 | R, T (B, C) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 8 | AA, BA, V, W, X... (L, O, B, C, E...) | water | water | 18.0 | 63 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 15 |
| Total formula weight | 441809.5 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 792.0 | |
| All* | Total formula weight | 442601.6 |
