8DH1
T7 RNA polymerase elongation complex with unnatural base dDs-PaTP pair
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 78.866, 86.156, 201.676 |
Unit cell angles | 89.85, 85.09, 69.56 |
Refinement procedure
Resolution | 39.340 - 2.650 |
R-factor | 0.2318 |
Rwork | 0.230 |
R-free | 0.27150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8dh0 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.697 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHENIX (1.19) |
Refinement software | PHENIX (1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.340 | 2.690 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.121 | 2.619 |
Rmeas | 0.140 | 3.036 |
Rpim | 0.071 | 1.533 |
Total number of observations | 509307 | 25182 |
Number of reflections | 130161 | 6486 |
<I/σ(I)> | 8.1 | 0.7 |
Completeness [%] | 90.3 | |
Redundancy | 3.9 | 3.9 |
CC(1/2) | 0.994 | 0.315 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 298 | 10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1 |