8DCG

Structure of guanylylated RNA ligase RtcB from Pyrococcus horikoshii

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	tRNA-splicing ligase RtcB	polymer	501	55753.8	2	UniProt (O59245) Pfam (PF01139)	Pyrococcus horikoshii OT3	3'-phosphate/5'-hydroxy nucleic acid ligase,tRNA ligase RtcB
2	C, D (C, D)	beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900003)
3	E, U (A, B)	GUANOSINE-5'-MONOPHOSPHATE	non-polymer		363.2	2	Chemie (5GP)
4	AA, BA, CA, DA, EA... (B, A)	SULFATE ION	non-polymer		96.1	25	Chemie (SO4)
5	HA, IA, S, T (B, A)	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
6	JA, KA (A, B)	water	water		18.0	73	Chemie (HOH)

Sequence modifications

A, B: 1 - 96 (UniProt: O59245)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

A, B: 97 - 481 (UniProt: O59245)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	111507.6
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	31
	Total formula weight	3269.8
All*	Total formula weight	115462.0

*Water molecules are not included.