8DCG

Structure of guanylylated RNA ligase RtcB from Pyrococcus horikoshii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-09
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.8917
Spacegroup name	P 43
Unit cell lengths	143.560, 143.560, 80.301
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.850 - 2.350
R-factor	0.1918
Rwork	0.191
R-free	0.21680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dwq
RMSD bond length	0.007
RMSD bond angle	0.962
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.850	2.400
High resolution limit [Å]	2.350	2.350
Rpim	0.033	0.341
Number of reflections	67599	4104
<I/σ(I)>	19.1	2.2
Completeness [%]	99.0	90.4
Redundancy	12.6	11
CC(1/2)	0.999	0.711

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	2.2 M ammonium sulfate cryoprotectant: 2.2 M ammonium sulfate, 22% (w/v) sucrose