8DCE

SARS-CoV-2 Receptor-Binding Domain SPEEDesign Immunogen 1 Bound to C144 scFv

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (H)	C144 scFv	polymer	276	28929.3	1		Homo sapiens (human)
2	B (A)	Spike protein S1	polymer	210	23639.3	1	UniProt (P0DTC2) Pfam (PF09408)	Severe acute respiratory syndrome coronavirus 2
3	C, D (H, A)	water	water		18.0	226	Chemie (HOH)

Sequence modifications

A: 333 - 526 (UniProt: P0DTC2)

PDB	External Database	Details
Glu 330	-	expression tag
Thr 331	-	expression tag
Gly 332	-	expression tag
Met 333	Thr 333	engineered mutation
Pro 348	Ala 348	engineered mutation
Tyr 362	Val 362	engineered mutation
Tyr 363	Ala 363	engineered mutation
Gln 394	Asn 394	engineered mutation
Phe 396	Tyr 396	engineered mutation
Thr 468	Ile 468	engineered mutation
Asp 519	His 519	engineered mutation
Pro 522	Ala 522	engineered mutation
Thr 527	-	expression tag
Gly 528	-	expression tag
Gly 529	-	expression tag
Gly 530	-	expression tag
Gly 531	-	expression tag
Ser 532	-	expression tag
Lys 533	-	expression tag
His 534	-	expression tag
His 535	-	expression tag
His 536	-	expression tag
His 537	-	expression tag
His 538	-	expression tag
His 539	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	52568.6
All*	Total formula weight	52568.6

*Water molecules are not included.