8D85

Cryo-EM structure of human IL-27 signaling complex: model containing the interaction core region

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	Interleukin-27 subunit beta	polymer	209	23338.7	1	UniProt (Q14213) Pfam (PF24031) Pfam (PF00041)	Homo sapiens (human)	IL-27 subunit beta,IL-27B,Epstein-Barr virus-induced gene 3 protein,EBV-induced gene 3 protein
2	B (D)	Interleukin-27 subunit alpha	polymer	243	27877.7	1	UniProt (Q8NEV9)	Homo sapiens (human)	IL-27 subunit alpha,IL-27-A,IL27-A,Interleukin-30,p28
3	C (A)	Interleukin-27 receptor subunit alpha	polymer	512	55944.2	1	UniProt (Q6UWB1)	Homo sapiens (human)	IL-27 receptor subunit alpha,IL-27R subunit alpha,IL-27R-alpha,IL-27RA,Cytokine receptor WSX-1,Cytokine receptor-like 1,Type I T-cell cytokine receptor,TCCR,ZcytoR1
4	D (B)	Interleukin-6 receptor subunit beta	polymer	625	71233.2	1	UniProt (P40189) Pfam (PF06328) Pfam (PF25552) Pfam (PF00041)	Homo sapiens (human)	IL-6 receptor subunit beta,IL-6R subunit beta,IL-6R-beta,IL-6RB,CDw130,Interleukin-6 signal transducer,Membrane glycoprotein 130,gp130,Oncostatin-M receptor subunit alpha
5	E, F, G (E, F, G)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
6	H, I, J, K, L (C, A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	5	Chemie (NAG)

Sequence modifications

D: 29 - 243 (UniProt: Q8NEV9)

PDB	External Database	Details
Glu 244	-	expression tag
Gln 245	-	expression tag
Lys 246	-	expression tag
Leu 247	-	expression tag
Ile 248	-	expression tag
Ser 249	-	expression tag
Glu 250	-	expression tag
Glu 251	-	expression tag
Asp 252	-	expression tag
Leu 253	-	expression tag
Gly 254	-	expression tag
Gly 255	-	expression tag
Glu 256	-	expression tag
Gln 257	-	expression tag
Lys 258	-	expression tag
Leu 259	-	expression tag
Ile 260	-	expression tag
Ser 261	-	expression tag
Glu 262	-	expression tag
Glu 263	-	expression tag
Asp 264	-	expression tag
Leu 265	-	expression tag
His 266	-	expression tag
His 267	-	expression tag
His 268	-	expression tag
His 269	-	expression tag
His 270	-	expression tag
His 271	-	expression tag

A: 33 - 516 (UniProt: Q6UWB1)

PDB	External Database	Details
Glu 517	-	expression tag
Gln 518	-	expression tag
Lys 519	-	expression tag
Leu 520	-	expression tag
Ile 521	-	expression tag
Ser 522	-	expression tag
Glu 523	-	expression tag
Glu 524	-	expression tag
Asp 525	-	expression tag
Leu 526	-	expression tag
Gly 527	-	expression tag
Gly 528	-	expression tag
Glu 529	-	expression tag
Gln 530	-	expression tag
Lys 531	-	expression tag
Leu 532	-	expression tag
Ile 533	-	expression tag
Ser 534	-	expression tag
Glu 535	-	expression tag
Glu 536	-	expression tag
Asp 537	-	expression tag
Leu 538	-	expression tag
His 539	-	expression tag
His 540	-	expression tag
His 541	-	expression tag
His 542	-	expression tag
His 543	-	expression tag
His 544	-	expression tag

B: 23 - 619 (UniProt: P40189)

PDB	External Database	Details
Glu 620	-	expression tag
Gln 621	-	expression tag
Lys 622	-	expression tag
Leu 623	-	expression tag
Ile 624	-	expression tag
Ser 625	-	expression tag
Glu 626	-	expression tag
Glu 627	-	expression tag
Asp 628	-	expression tag
Leu 629	-	expression tag
Gly 630	-	expression tag
Gly 631	-	expression tag
Glu 632	-	expression tag
Gln 633	-	expression tag
Lys 634	-	expression tag
Leu 635	-	expression tag
Ile 636	-	expression tag
Ser 637	-	expression tag
Glu 638	-	expression tag
Glu 639	-	expression tag
Asp 640	-	expression tag
Leu 641	-	expression tag
His 642	-	expression tag
His 643	-	expression tag
His 644	-	expression tag
His 645	-	expression tag
His 646	-	expression tag
His 647	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	178393.8
Branched	Number of molecules	3
	Total formula weight	1273.2
Non-Polymers*	Number of molecules	5
	Total formula weight	1106.0
All*	Total formula weight	180773.1

*Water molecules are not included.