8D7H

Cryo-EM structure of human CLCF1 in complex with CRLF1 and CNTFR alpha

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (C, G)	Ciliary neurotrophic factor receptor subunit alpha	polymer	348	39170.8	2	UniProt (P26992) Pfam (PF00041)	Homo sapiens (human)	CNTF receptor subunit alpha,CNTFR-alpha
2	B, E (D, H)	Cardiotrophin-like cytokine factor 1	polymer	204	23122.3	2	UniProt (Q9UBD9) Pfam (PF06875)	Homo sapiens (human)	B-cell-stimulating factor 3,BSF-3,Novel neurotrophin-1,NNT-1
3	C, F (A, E)	Cytokine receptor-like factor 1	polymer	395	44026.7	2	UniProt (O75462) Pfam (PF09067) Pfam (PF00041)	Homo sapiens (human)	Cytokine-like factor 1,CLF-1,ZcytoR5
4	G, H, I, J, K... (B, F, I, J, K...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	14	In PDB GlyTouCan (G42666HT)
5	U, V, W, X (A, E)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)

Sequence modifications

C, G: 23 - 342 (UniProt: P26992)

PDB	External Database	Details
Glu 343	-	expression tag
Gln 344	-	expression tag
Lys 345	-	expression tag
Leu 346	-	expression tag
Ile 347	-	expression tag
Ser 348	-	expression tag
Glu 349	-	expression tag
Glu 350	-	expression tag
Asp 351	-	expression tag
Leu 352	-	expression tag
Gly 353	-	expression tag
Gly 354	-	expression tag
Glu 355	-	expression tag
Gln 356	-	expression tag
Lys 357	-	expression tag
Leu 358	-	expression tag
Ile 359	-	expression tag
Ser 360	-	expression tag
Glu 361	-	expression tag
Glu 362	-	expression tag
Asp 363	-	expression tag
Leu 364	-	expression tag
His 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag

D, H: 28 - 225 (UniProt: Q9UBD9)

PDB	External Database	Details
His 226	-	expression tag
His 227	-	expression tag
His 228	-	expression tag
His 229	-	expression tag
His 230	-	expression tag
His 231	-	expression tag

A, E: 38 - 422 (UniProt: O75462)

PDB	External Database	Details
His 423	-	expression tag
His 424	-	expression tag
His 425	-	expression tag
His 426	-	expression tag
His 427	-	expression tag
His 428	-	expression tag
His 429	-	expression tag
His 430	-	expression tag
His 431	-	expression tag
His 432	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	212639.7
Branched	Number of molecules	14
	Total formula weight	5941.6
Non-Polymers*	Number of molecules	4
	Total formula weight	884.8
All*	Total formula weight	219466.1

*Water molecules are not included.