8D7E

Cryo-EM structure of human CNTFR alpha in complex with the Fab fragments of two antibodies

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	Ciliary neurotrophic factor receptor subunit alpha	polymer	348	39170.8	1	UniProt (P26992) Pfam (PF00041)	Homo sapiens (human)	CNTF receptor subunit alpha,CNTFR-alpha
2	B (G)	H4H25311P2 antibody Fab fragment light chain	polymer	215	23447.0	1		Homo sapiens
3	C (F)	H4H25311P2 antibody Fab fragment heavy chain	polymer	217	23529.4	1		Homo sapiens
4	D (E)	REGN8938 antibody Fab fragment light chain	polymer	215	23439.0	1		Homo sapiens
5	E (D)	REGN8938 antibody Fab fragment heavy chain	polymer	220	23922.7	1		Homo sapiens
6	F, G, H, I (A, B, H, I)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)

Sequence modifications

C: 23 - 342 (UniProt: P26992)

PDB	External Database	Details
Glu 343	-	expression tag
Gln 344	-	expression tag
Lys 345	-	expression tag
Leu 346	-	expression tag
Ile 347	-	expression tag
Ser 348	-	expression tag
Glu 349	-	expression tag
Glu 350	-	expression tag
Asp 351	-	expression tag
Leu 352	-	expression tag
Gly 353	-	expression tag
Gly 354	-	expression tag
Glu 355	-	expression tag
Gln 356	-	expression tag
Lys 357	-	expression tag
Leu 358	-	expression tag
Ile 359	-	expression tag
Ser 360	-	expression tag
Glu 361	-	expression tag
Glu 362	-	expression tag
Asp 363	-	expression tag
Leu 364	-	expression tag
His 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	133508.9
Branched	Number of molecules	4
	Total formula weight	1697.6
All*	Total formula weight	135206.5

*Water molecules are not included.