8D6A

Cryo-EM structure of human LIF signaling complex: model containing the interaction core region

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	D	Leukemia inhibitory factor	polymer	179	19652.8	1	UniProt (P15018) Pfam (PF01291) In PDB	Homo sapiens (human)	LIF,Differentiation-stimulating factor,D factor,Melanoma-derived LPL inhibitor,MLPLI
2	A	Interleukin-6 receptor subunit beta	polymer	625	71233.2	1	UniProt (P40189) Pfam (PF06328) Pfam (PF09240) Pfam (PF00041) In PDB	Homo sapiens (human)	IL-6 receptor subunit beta,IL-6R subunit beta,IL-6R-beta,IL-6RB,CDw130,Interleukin-6 signal transducer,Membrane glycoprotein 130,gp130,Oncostatin-M receptor subunit alpha
3	B	Leukemia inhibitory factor receptor	polymer	817	92834.8	1	UniProt (P42702) Pfam (PF18207) Pfam (PF17971) Pfam (PF21177) Pfam (PF00041) In PDB	Homo sapiens (human)	LIF receptor,LIF-R
4	C, E, F, G, H	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	5	In PDB GlyTouCan (G42666HT)
5	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	5	Chemie (NAG)

Sequence modifications

A: 23 - 619 (UniProt: P40189)

PDB	External Database	Details
Glu 620	-	expression tag
Gln 621	-	expression tag
Lys 622	-	expression tag
Leu 623	-	expression tag
Ile 624	-	expression tag
Ser 625	-	expression tag
Glu 626	-	expression tag
Glu 627	-	expression tag
Asp 628	-	expression tag
Leu 629	-	expression tag
Gly 630	-	expression tag
Gly 631	-	expression tag
Glu 632	-	expression tag
Gln 633	-	expression tag
Lys 634	-	expression tag
Leu 635	-	expression tag
Ile 636	-	expression tag
Ser 637	-	expression tag
Glu 638	-	expression tag
Glu 639	-	expression tag
Asp 640	-	expression tag
Leu 641	-	expression tag
His 642	-	expression tag
His 643	-	expression tag
His 644	-	expression tag
His 645	-	expression tag
His 646	-	expression tag
His 647	-	expression tag

B: 45 - 833 (UniProt: P42702)

PDB	External Database	Details
Glu 834	-	expression tag
Gln 835	-	expression tag
Lys 836	-	expression tag
Leu 837	-	expression tag
Ile 838	-	expression tag
Ser 839	-	expression tag
Glu 840	-	expression tag
Glu 841	-	expression tag
Asp 842	-	expression tag
Leu 843	-	expression tag
Gly 844	-	expression tag
Gly 845	-	expression tag
Glu 846	-	expression tag
Gln 847	-	expression tag
Lys 848	-	expression tag
Leu 849	-	expression tag
Ile 850	-	expression tag
Ser 851	-	expression tag
Glu 852	-	expression tag
Glu 853	-	expression tag
Asp 854	-	expression tag
Leu 855	-	expression tag
His 856	-	expression tag
His 857	-	expression tag
His 858	-	expression tag
His 859	-	expression tag
His 860	-	expression tag
His 861	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	183720.8
Branched	Number of molecules	5
	Total formula weight	2122.0
Non-Polymers*	Number of molecules	5
	Total formula weight	1106.0
All*	Total formula weight	186948.8

*Water molecules are not included.