8D4D
gamma-Arf1 mediated dimeric assembly of AP-1, Arf1, Nef complex within lattice on MHC-I lipopeptide incorporated narrow membrane tubes
This is a non-PDB format compatible entry.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | C, H, D, F | ADP-ribosylation factor 1 | polymer | 180 | 20590.5 | 4 | UniProt (P84077) Pfam (PF00025) In PDB | Homo sapiens (human) | |
2 | N, L, K, I | Protein Nef | polymer | 212 | 24154.0 | 4 | UniProt (Q90VU7) Pfam (PF00469) In PDB | Human immunodeficiency virus 1 | 3'ORF,Negative factor,F-protein |
3 | Y, P | HLA class I histocompatibility antigen, A alpha chain | polymer | 39 | 4139.4 | 2 | UniProt (P04439) Pfam (PF06623) In PDB | Homo sapiens (human) | Human leukocyte antigen A,HLA-A |
4 | B, A | AP-1 complex subunit beta-1 | polymer | 949 | 104736.5 | 2 | UniProt (Q10567) Pfam (PF01602) Pfam (PF02883) Pfam (PF09066) In PDB | Homo sapiens (human) | Adaptor protein complex AP-1 subunit beta-1,Adaptor-related protein complex 1 subunit beta-1,Beta-1-adaptin,Beta-adaptin 1,Clathrin assembly protein complex 1 beta large chain,Golgi adaptor HA1/AP1 adaptin beta subunit |
5 | G, E | AP-1 complex subunit gamma-1 | polymer | 601 | 68194.1 | 2 | UniProt (P22892) Pfam (PF01602) In PDB | Mus musculus (house mouse) | Adaptor protein complex AP-1 subunit gamma-1,Adaptor-related protein complex 1 subunit gamma-1,Clathrin assembly protein complex 1 gamma-1 large chain,Gamma-adaptin,Gamma1-adaptin,Golgi adaptor HA1/AP1 adaptin subunit gamma-1 |
6 | M, J | AP-1 complex subunit mu-1 | polymer | 423 | 48606.7 | 2 | UniProt (P35585) Pfam (PF01217) Pfam (PF00928) In PDB | Mus musculus (house mouse) | AP-mu chain family member mu1A,Adaptor protein complex AP-1 subunit mu-1,Adaptor-related protein complex 1 subunit mu-1,Clathrin assembly protein complex 1 mu-1 medium chain 1,Clathrin coat assembly protein AP47,Clathrin coat-associated protein AP47,Golgi adaptor HA1/AP1 adaptin mu-1 subunit,Mu-adaptin 1,Mu1A-adaptin |
7 | S, O | AP-1 complex subunit sigma-3 | polymer | 154 | 18305.3 | 2 | UniProt (Q96PC3) Pfam (PF01217) In PDB | Homo sapiens (human) | Adaptor protein complex AP-1 subunit sigma-1C,Adaptor-related protein complex 1 subunit sigma-1C,Clathrin assembly protein complex 1 sigma-1C small chain,Golgi adaptor HA1/AP1 adaptin sigma-1C subunit,Sigma 1C subunit of AP-1 clathrin,Sigma-adaptin 1C,Sigma1C-adaptin |
8 | C, H, D, F | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 4 | Chemie (GTP) | |||
9 | C, H, D, F | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) |
Sequence modifications
N, L, K, I: 2 - 206 (UniProt: Q90VU7)
Y, P: 334 - 365 (UniProt: P04439)
B, A: 1 - 949 (UniProt: Q10567)
G, E: 1 - 595 (UniProt: P22892)
PDB | External Database | Details |
---|---|---|
Gly 207 | - | expression tag |
His 208 | - | expression tag |
His 209 | - | expression tag |
His 210 | - | expression tag |
His 211 | - | expression tag |
His 212 | - | expression tag |
His 213 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Cys 333 | - | expression tag |
Ser 345 | Thr 345 | engineered mutation |
Gly 349 | Ser 349 | engineered mutation |
Ser 355 | Gly 355 | engineered mutation |
Ala 363 | Cys 363 | engineered mutation |
His 366 | - | expression tag |
His 367 | - | expression tag |
His 368 | - | expression tag |
His 369 | - | expression tag |
His 370 | - | expression tag |
His 371 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Arg 359 | Lys 359 | engineered mutation |
Lys 476 | Glu 476 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Glu 596 | - | expression tag |
Asn 597 | - | expression tag |
Leu 598 | - | expression tag |
Tyr 599 | - | expression tag |
Phe 600 | - | expression tag |
Gln 601 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 18 |
Total formula weight | 666942.4 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 2189.9 | |
All* | Total formula weight | 669132.3 |