8D3P

Type I-C Cas4-Cas1-Cas2 complex bound to half-site integration intermediate (HSI)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	CRISPR-associated endonuclease Cas1	polymer	347	39665.6	4	UniProt (Q9KFX9) Pfam (PF01867)	Alkalihalobacillus halodurans C-125
2	E, F (E, F)	CRISPR-associated endonuclease Cas2	polymer	100	11166.8	2	UniProt (Q9KFX8) Pfam (PF09827)	Alkalihalobacillus halodurans C-125
3	G (G)	HSI strand 2- integrated prespacer strand plus repeat	polymer	58	17915.4	1		synthetic construct
4	H (H)	HSI strand 2	polymer	36	11048.1	1		synthetic construct
5	I (I)	CRISPR-associated exonuclease Cas4	polymer	218	25403.4	1	UniProt (A0A4Y7WTW2)	Alkalihalobacillus halodurans C-125
6	J (K)	HSI strand 3 bottom strand leader-repeat	polymer	41	12583.1	1		synthetic construct
7	K (L)	HSI strand 4 top leader strand	polymer	11	3355.2	1		synthetic construct
8	L (I)	IRON/SULFUR CLUSTER	non-polymer		351.6	1	Chemie (SF4)
9	M (I)	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)

Sequence modifications

A, B, C, D: 1 - 343 (UniProt: Q9KFX9)

PDB	External Database	Details
Gly -3	-	expression tag
Ala -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

E, F: 1 - 96 (UniProt: Q9KFX8)

PDB	External Database	Details
Gly -3	-	expression tag
Ala -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

I: 3 - 219 (UniProt: A0A4Y7WTW2)

PDB	External Database	Details
Ala 2	-	expression tag
Met 11	Leu 11	conflict
Ser 101	Cys 101	conflict

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	251301.2
Non-Polymers*	Number of molecules	2
	Total formula weight	406.6
All*	Total formula weight	251707.8

*Water molecules are not included.