8D21

Cryo-EM structure of the VRC321 clinical trial, vaccine-elicited, human antibody 1B06 in complex with a stabilized NC99 HA trimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I (B, C, G)	Hemagglutinin HA2 chain	polymer	222	25263.1	3	UniProt (Q289M7) Pfam (PF00509) UniProt (by SIFTS) (Q6WG00)	Influenza A virus
2	B, F, J (A, D, I)	Hemagglutinin HA1 chain	polymer	326	36407.9	3	UniProt (Q6WG00) Pfam (PF00509)	Influenza A virus
3	C, G, K (H, E, J)	1B06 Heavy Chain	polymer	123	13499.1	3		Homo sapiens
4	D, H, L (L, F, K)	1B06 Light Chain	polymer	108	11778.1	3		Homo sapiens
5	M, N, O (M, N, O)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
6	AA, BA, CA, DA, EA... (D, I, A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	21	Chemie (NAG)

Sequence modifications

B, C, G: 1 - 176 (UniProt: Q289M7)

PDB	External Database	Details
Cys 47	Gly 390	conflict
Ser 177	-	expression tag
Gly 178	-	expression tag
Arg 179	-	expression tag
Leu 180	-	expression tag
Val 181	-	expression tag
Pro 182	-	expression tag
Arg 183	-	expression tag
Gly 184	-	expression tag
Ser 185	-	expression tag
Pro 186	-	expression tag
Gly 187	-	expression tag
Ser 188	-	expression tag
Gly 189	-	expression tag
Tyr 190	-	expression tag
Ile 191	-	expression tag
Pro 192	-	expression tag
Glu 193	-	expression tag
Ala 194	-	expression tag
Pro 195	-	expression tag
Arg 196	-	expression tag
Asp 197	-	expression tag
Gly 198	-	expression tag
Gln 199	-	expression tag
Ala 200	-	expression tag
Tyr 201	-	expression tag
Val 202	-	expression tag
Arg 203	-	expression tag
Lys 204	-	expression tag
Asp 205	-	expression tag
Gly 206	-	expression tag
Glu 207	-	expression tag
Trp 208	-	expression tag
Val 209	-	expression tag
Leu 210	-	expression tag
Leu 211	-	expression tag
Ser 212	-	expression tag
Thr 213	-	expression tag
Phe 214	-	expression tag
Leu 215	-	expression tag
Gly 216	-	expression tag
His 217	-	expression tag
His 218	-	expression tag
His 219	-	expression tag
His 220	-	expression tag
His 221	-	expression tag
His 222	-	expression tag

A, D, I: 11 - 329 (UniProt: Q6WG00)

PDB	External Database	Details
Cys 30	Leu 37	conflict

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	260844.5
Branched	Number of molecules	3
	Total formula weight	1759.6
Non-Polymers*	Number of molecules	21
	Total formula weight	4645.4
All*	Total formula weight	267249.5

*Water molecules are not included.