8CU0
12-mer DNA structure of ExBIM bound to RNaseH -modified DDD
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C | Ribonuclease H | polymer | 142 | 16329.5 | 3 | UniProt (Q9KEI9) In PDB | Halalkalibacterium halodurans | RNase H |
| 2 | D, E | DNA (5'-D(*CP*GP*GP*GP*CP*AP*TP*GP*(OWR)P*CP*CP*G)-3') | polymer | 12 | 3721.5 | 2 | synthetic construct | ||
| 3 | A, B, C | 1,2-ETHANEDIOL | non-polymer | 62.1 | 5 | Chemie (EDO) | |||
| 4 | A, B, C | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 5 | A, B, C | SODIUM ION | non-polymer | 23.0 | 4 | Chemie (NA) | |||
| 6 | water | water | 18.0 | 228 | Chemie (HOH) |
Sequence modifications
A, B, C: 59 - 196 (UniProt: Q9KEI9)
| PDB | External Database | Details |
|---|---|---|
| Gly 55 | - | expression tag |
| Ser 56 | - | expression tag |
| His 57 | - | expression tag |
| Met 58 | - | expression tag |
| Asn 132 | Asp 132 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 56431.4 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 678.6 | |
| All* | Total formula weight | 57110.0 |
