8CU0
12-mer DNA structure of ExBIM bound to RNaseH -modified DDD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2018-07-11 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.894, 89.403, 73.890 |
Unit cell angles | 90.00, 101.42, 90.00 |
Refinement procedure
Resolution | 28.490 - 1.740 |
R-factor | 0.2045 |
Rwork | 0.202 |
R-free | 0.24630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3EY1 (Protein alone) |
RMSD bond length | 0.008 |
RMSD bond angle | 1.018 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.600 | 1.810 |
High resolution limit [Å] | 1.740 | 1.740 |
Rmerge | 0.106 | 1.585 |
Rmeas | 1.749 | |
Rpim | 0.040 | 0.716 |
Number of reflections | 47425 | 4470 |
<I/σ(I)> | 22.11 | 0.63 |
Completeness [%] | 99.3 | 93.9 |
Redundancy | 7.8 | |
CC(1/2) | 0.327 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.2 M sodium acetate trihydrate, 0.1 M sodium cacodylate trihydrate, 30% PEG 8000 (W/V) |