8CTZ
12-mer DNA structure of ExBIM & O6Me-G bound to RNase-H
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | Ribonuclease H | polymer | 142 | 16329.5 | 3 | UniProt (Q9KEI9) | Alkalihalobacillus halodurans | RNase H |
| 2 | D, E (D, E) | DNA (5'-D(*CP*GP*CP*(6OG)P*AP*AP*TP*TP*(OWR)P*GP*CP*G)-3') | polymer | 12 | 3734.5 | 2 | synthetic construct | ||
| 3 | F, G, I, L (A, B, C) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 4 | H, K, N (A, B, C) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 5 | J, M (B, C) | ACETATE ION | non-polymer | 59.0 | 2 | Chemie (ACT) | |||
| 6 | O (C) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 7 | P, Q (C, D) | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) | |||
| 8 | R, S, T, U, V (A, B, C, D, E) | water | water | 18.0 | 119 | Chemie (HOH) |
Sequence modifications
A, B, C: 59 - 196 (UniProt: Q9KEI9)
| PDB | External Database | Details |
|---|---|---|
| Gly 55 | - | expression tag |
| Ser 56 | - | expression tag |
| His 57 | - | expression tag |
| Met 58 | - | expression tag |
| Asn 132 | Asp 132 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 56457.5 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 773.9 | |
| All* | Total formula weight | 57231.3 |
