8CTZ

12-mer DNA structure of ExBIM & O6Me-G bound to RNase-H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-07-11
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97856
Spacegroup name	P 1 21 1
Unit cell lengths	59.506, 66.492, 69.182
Unit cell angles	90.00, 107.97, 90.00

Refinement procedure

Resolution	29.740 - 2.320
R-factor	0.1988
Rwork	0.195
R-free	0.26510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3EY1 (Protein alone)
RMSD bond length	0.008
RMSD bond angle	1.043
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.750	2.360
High resolution limit [Å]	2.320	2.320
Rmerge	0.121	0.620
Rpim	0.062
Number of reflections	22274	1062
<I/σ(I)>	14.63	1.8
Completeness [%]	99.4	95.7
Redundancy	4.9	4.3
CC(1/2)		0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.1 M HEPES, 10% v/v 2-propanol and 20% w/v PEG 4000