8CRB

Cryo-EM structure of PcrV/Fab(11-E5)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Heavy chain	polymer	227	24036.7	1		Homo sapiens
2	B (B)	Light chain	polymer	216	22964.4	1		Homo sapiens
3	C (C)	Maltose/maltodextrin-binding periplasmic protein,Type III secretion protein PcrV	polymer	692	75538.1	1	UniProt (P0AEX9) UniProt (G3XD49) Pfam (PF01547) Pfam (PF04792)	Pseudomonas aeruginosa	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP

Sequence modifications

C: -354 - 1 (UniProt: P0AEX9)

PDB	External Database	Details
Met -397	-	initiating methionine
Gly -396	-	expression tag
Ser -395	-	expression tag
Trp -394	-	expression tag
Ser -393	-	expression tag
His -392	-	expression tag
Pro -391	-	expression tag
Gln -390	-	expression tag
Phe -389	-	expression tag
Glu -388	-	expression tag
Lys -387	-	expression tag
Gly -386	-	expression tag
Gly -385	-	expression tag
Gly -384	-	expression tag
Ser -383	-	expression tag
Gly -382	-	expression tag
Gly -381	-	expression tag
Gly -380	-	expression tag
Ser -379	-	expression tag
Gly -378	-	expression tag
Gly -377	-	expression tag
Gly -376	-	expression tag
Ser -375	-	expression tag
Trp -374	-	expression tag
Ser -373	-	expression tag
His -372	-	expression tag
Pro -371	-	expression tag
Gln -370	-	expression tag
Phe -369	-	expression tag
Glu -368	-	expression tag
Lys -367	-	expression tag
Ser -366	-	expression tag
Gly -365	-	expression tag
Leu -364	-	expression tag
Val -363	-	expression tag
Pro -362	-	expression tag
Arg -361	-	expression tag
Gly -360	-	expression tag
Ser -359	-	expression tag
Ala -358	-	expression tag
Ser -357	-	expression tag
Lys -356	-	expression tag
Thr -355	-	expression tag
Ala -275	Asp 108	conflict
Ala -274	Lys 109	conflict
Ala -185	Glu 198	conflict
Ala -184	Asn 199	conflict
His -142	Ala 241	conflict
His -138	Lys 245	conflict
Val -45	Ala 338	conflict
Val -40	Ile 343	conflict

C: 2 - 294 (UniProt: G3XD49)

PDB	External Database	Details
Pro 21	Ser 21	conflict
Lys 225	Ser 225	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	122539.2
All*	Total formula weight	122539.2

*Water molecules are not included.