8CHF

cryo-EM Structure of Craf:14-3-3:Mek1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	RAF proto-oncogene serine/threonine-protein kinase	polymer	648	73184.5	2	UniProt (P04049) Pfam (PF02196) Pfam (PF00130) Pfam (PF00069)	Homo sapiens (human)	Proto-oncogene c-RAF,cRaf,Raf-1
2	C, D (C, D)	14-3-3 protein zeta isoform X1	polymer	247	28108.5	2	UniProt (A0A9R0D7T1) Pfam (PF00244)	Spodoptera frugiperda (fall armyworm)
3	E, F (E, F)	Dual specificity mitogen-activated protein kinase kinase 1	polymer	393	43461.9	2	UniProt (Q02750) Pfam (PF00069)	Homo sapiens (human)	MAP kinase kinase 1,MAPKK 1,MKK1,ERK activator kinase 1,MAPK/ERK kinase 1,MEK 1
4	G, H (A, B)	2-{4-[(1E)-1-(hydroxyimino)-2,3-dihydro-1H-inden-5-yl]-3-(pyridin-4-yl)-1H-pyrazol-1-yl}ethanol	non-polymer		334.4	2	Chemie (29L)

Sequence modifications

A, B: 1 - 648 (UniProt: P04049)

PDB	External Database	Details
Asp 341	Tyr 341	engineered mutation

E, F: 1 - 393 (UniProt: Q02750)

PDB	External Database	Details
Ala 218	Ser 218	engineered mutation
Ala 222	Ser 222	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	289509.9
Non-Polymers*	Number of molecules	2
	Total formula weight	668.7
All*	Total formula weight	290178.6

*Water molecules are not included.