8CHF
cryo-EM Structure of Craf:14-3-3:Mek1
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | RAF proto-oncogene serine/threonine-protein kinase | polymer | 648 | 73184.5 | 2 | UniProt (P04049) Pfam (PF02196) Pfam (PF00130) Pfam (PF00069) In PDB | Homo sapiens (human) | Proto-oncogene c-RAF,cRaf,Raf-1 |
2 | C, D | 14-3-3 protein zeta isoform X1 | polymer | 247 | 28108.5 | 2 | UniProt (A0A9R0D7T1) Pfam (PF00244) In PDB | Spodoptera frugiperda (fall armyworm) | |
3 | E, F | Dual specificity mitogen-activated protein kinase kinase 1 | polymer | 393 | 43461.9 | 2 | UniProt (Q02750) Pfam (PF00069) In PDB | Homo sapiens (human) | MAP kinase kinase 1,MAPKK 1,MKK1,ERK activator kinase 1,MAPK/ERK kinase 1,MEK 1 |
4 | A, B | 2-{4-[(1E)-1-(hydroxyimino)-2,3-dihydro-1H-inden-5-yl]-3-(pyridin-4-yl)-1H-pyrazol-1-yl}ethanol | non-polymer | 334.4 | 2 | Chemie (29L) |
Sequence modifications
A, B: 1 - 648 (UniProt: P04049)
E, F: 1 - 393 (UniProt: Q02750)
PDB | External Database | Details |
---|---|---|
Asp 341 | Tyr 341 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Ala 218 | Ser 218 | engineered mutation |
Ala 222 | Ser 222 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 289509.9 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 668.7 | |
All* | Total formula weight | 290178.6 |