8CHF

cryo-EM Structure of Craf:14-3-3:Mek1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000165	biological_process	MAPK cascade
A	0000166	molecular_function	nucleotide binding
A	0004672	molecular_function	protein kinase activity
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0004709	molecular_function	MAP kinase kinase kinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005741	cellular_component	mitochondrial outer membrane
A	0005794	cellular_component	Golgi apparatus
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006468	biological_process	protein phosphorylation
A	0006915	biological_process	apoptotic process
A	0007165	biological_process	signal transduction
A	0007528	biological_process	neuromuscular junction development
A	0008270	molecular_function	zinc ion binding
A	0008285	biological_process	negative regulation of cell population proliferation
A	0008286	biological_process	insulin receptor signaling pathway
A	0010856	molecular_function	adenylate cyclase activator activity
A	0014044	biological_process	Schwann cell development
A	0016301	molecular_function	kinase activity
A	0016740	molecular_function	transferase activity
A	0019899	molecular_function	enzyme binding
A	0031143	cellular_component	pseudopodium
A	0031267	molecular_function	small GTPase binding
A	0031333	biological_process	negative regulation of protein-containing complex assembly
A	0033138	biological_process	positive regulation of peptidyl-serine phosphorylation
A	0035023	biological_process	regulation of Rho protein signal transduction
A	0038127	biological_process	ERBB signaling pathway
A	0038133	biological_process	ERBB2-ERBB3 signaling pathway
A	0042060	biological_process	wound healing
A	0042552	biological_process	myelination
A	0042802	molecular_function	identical protein binding
A	0042981	biological_process	regulation of apoptotic process
A	0043066	biological_process	negative regulation of apoptotic process
A	0043410	biological_process	positive regulation of MAPK cascade
A	0045595	biological_process	regulation of cell differentiation
A	0046872	molecular_function	metal ion binding
A	0048009	biological_process	insulin-like growth factor receptor signaling pathway
A	0060333	biological_process	type II interferon-mediated signaling pathway
A	0106310	molecular_function	protein serine kinase activity
B	0000165	biological_process	MAPK cascade
B	0000166	molecular_function	nucleotide binding
B	0004672	molecular_function	protein kinase activity
B	0004674	molecular_function	protein serine/threonine kinase activity
B	0004709	molecular_function	MAP kinase kinase kinase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005741	cellular_component	mitochondrial outer membrane
B	0005794	cellular_component	Golgi apparatus
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006468	biological_process	protein phosphorylation
B	0006915	biological_process	apoptotic process
B	0007165	biological_process	signal transduction
B	0007528	biological_process	neuromuscular junction development
B	0008270	molecular_function	zinc ion binding
B	0008285	biological_process	negative regulation of cell population proliferation
B	0008286	biological_process	insulin receptor signaling pathway
B	0010856	molecular_function	adenylate cyclase activator activity
B	0014044	biological_process	Schwann cell development
B	0016301	molecular_function	kinase activity
B	0016740	molecular_function	transferase activity
B	0019899	molecular_function	enzyme binding
B	0031143	cellular_component	pseudopodium
B	0031267	molecular_function	small GTPase binding
B	0031333	biological_process	negative regulation of protein-containing complex assembly
B	0033138	biological_process	positive regulation of peptidyl-serine phosphorylation
B	0035023	biological_process	regulation of Rho protein signal transduction
B	0038127	biological_process	ERBB signaling pathway
B	0038133	biological_process	ERBB2-ERBB3 signaling pathway
B	0042060	biological_process	wound healing
B	0042552	biological_process	myelination
B	0042802	molecular_function	identical protein binding
B	0042981	biological_process	regulation of apoptotic process
B	0043066	biological_process	negative regulation of apoptotic process
B	0043410	biological_process	positive regulation of MAPK cascade
B	0045595	biological_process	regulation of cell differentiation
B	0046872	molecular_function	metal ion binding
B	0048009	biological_process	insulin-like growth factor receptor signaling pathway
B	0060333	biological_process	type II interferon-mediated signaling pathway
B	0106310	molecular_function	protein serine kinase activity
E	0000165	biological_process	MAPK cascade
E	0000166	molecular_function	nucleotide binding
E	0000187	biological_process	obsolete activation of MAPK activity
E	0003056	biological_process	regulation of vascular associated smooth muscle contraction
E	0004672	molecular_function	protein kinase activity
E	0004674	molecular_function	protein serine/threonine kinase activity
E	0004708	molecular_function	MAP kinase kinase activity
E	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
E	0004713	molecular_function	protein tyrosine kinase activity
E	0005078	molecular_function	MAP-kinase scaffold activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005739	cellular_component	mitochondrion
E	0005769	cellular_component	early endosome
E	0005770	cellular_component	late endosome
E	0005783	cellular_component	endoplasmic reticulum
E	0005794	cellular_component	Golgi apparatus
E	0005813	cellular_component	centrosome
E	0005829	cellular_component	cytosol
E	0005874	cellular_component	microtubule
E	0005886	cellular_component	plasma membrane
E	0005925	cellular_component	focal adhesion
E	0005938	cellular_component	cell cortex
E	0006468	biological_process	protein phosphorylation
E	0006935	biological_process	chemotaxis
E	0006979	biological_process	response to oxidative stress
E	0007165	biological_process	signal transduction
E	0007528	biological_process	neuromuscular junction development
E	0008285	biological_process	negative regulation of cell population proliferation
E	0010508	biological_process	positive regulation of autophagy
E	0010628	biological_process	positive regulation of gene expression
E	0010629	biological_process	negative regulation of gene expression
E	0014044	biological_process	Schwann cell development
E	0014069	cellular_component	postsynaptic density
E	0016020	cellular_component	membrane
E	0016301	molecular_function	kinase activity
E	0016740	molecular_function	transferase activity
E	0019901	molecular_function	protein kinase binding
E	0030182	biological_process	neuron differentiation
E	0030295	molecular_function	protein kinase activator activity
E	0030335	biological_process	positive regulation of cell migration
E	0030424	cellular_component	axon
E	0030425	cellular_component	dendrite
E	0031267	molecular_function	small GTPase binding
E	0031435	molecular_function	mitogen-activated protein kinase kinase kinase binding
E	0032402	biological_process	melanosome transport
E	0032839	cellular_component	dendrite cytoplasm
E	0032872	biological_process	regulation of stress-activated MAPK cascade
E	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
E	0034111	biological_process	negative regulation of homotypic cell-cell adhesion
E	0036064	cellular_component	ciliary basal body
E	0038127	biological_process	ERBB signaling pathway
E	0038133	biological_process	ERBB2-ERBB3 signaling pathway
E	0042552	biological_process	myelination
E	0043204	cellular_component	perikaryon
E	0043410	biological_process	positive regulation of MAPK cascade
E	0043539	molecular_function	protein serine/threonine kinase activator activity
E	0044877	molecular_function	protein-containing complex binding
E	0045893	biological_process	positive regulation of DNA-templated transcription
E	0045933	biological_process	positive regulation of muscle contraction
E	0047496	biological_process	vesicle transport along microtubule
E	0048009	biological_process	insulin-like growth factor receptor signaling pathway
E	0048313	biological_process	Golgi inheritance
E	0048471	cellular_component	perinuclear region of cytoplasm
E	0048678	biological_process	response to axon injury
E	0048812	biological_process	neuron projection morphogenesis
E	0051384	biological_process	response to glucocorticoid
E	0070328	biological_process	triglyceride homeostasis
E	0070371	biological_process	ERK1 and ERK2 cascade
E	0070372	biological_process	regulation of ERK1 and ERK2 cascade
E	0070374	biological_process	positive regulation of ERK1 and ERK2 cascade
E	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
E	0090170	biological_process	regulation of Golgi inheritance
E	0090398	biological_process	cellular senescence
E	0097110	molecular_function	scaffold protein binding
E	0098696	biological_process	regulation of neurotransmitter receptor localization to postsynaptic specialization membrane
E	0098978	cellular_component	glutamatergic synapse
E	0106310	molecular_function	protein serine kinase activity
E	1903298	biological_process	negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
E	2000641	biological_process	regulation of early endosome to late endosome transport
E	2001171	biological_process	positive regulation of ATP biosynthetic process
F	0000165	biological_process	MAPK cascade
F	0000166	molecular_function	nucleotide binding
F	0000187	biological_process	obsolete activation of MAPK activity
F	0003056	biological_process	regulation of vascular associated smooth muscle contraction
F	0004672	molecular_function	protein kinase activity
F	0004674	molecular_function	protein serine/threonine kinase activity
F	0004708	molecular_function	MAP kinase kinase activity
F	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
F	0004713	molecular_function	protein tyrosine kinase activity
F	0005078	molecular_function	MAP-kinase scaffold activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005739	cellular_component	mitochondrion
F	0005769	cellular_component	early endosome
F	0005770	cellular_component	late endosome
F	0005783	cellular_component	endoplasmic reticulum
F	0005794	cellular_component	Golgi apparatus
F	0005813	cellular_component	centrosome
F	0005829	cellular_component	cytosol
F	0005874	cellular_component	microtubule
F	0005886	cellular_component	plasma membrane
F	0005925	cellular_component	focal adhesion
F	0005938	cellular_component	cell cortex
F	0006468	biological_process	protein phosphorylation
F	0006935	biological_process	chemotaxis
F	0006979	biological_process	response to oxidative stress
F	0007165	biological_process	signal transduction
F	0007528	biological_process	neuromuscular junction development
F	0008285	biological_process	negative regulation of cell population proliferation
F	0010508	biological_process	positive regulation of autophagy
F	0010628	biological_process	positive regulation of gene expression
F	0010629	biological_process	negative regulation of gene expression
F	0014044	biological_process	Schwann cell development
F	0014069	cellular_component	postsynaptic density
F	0016020	cellular_component	membrane
F	0016301	molecular_function	kinase activity
F	0016740	molecular_function	transferase activity
F	0019901	molecular_function	protein kinase binding
F	0030182	biological_process	neuron differentiation
F	0030295	molecular_function	protein kinase activator activity
F	0030335	biological_process	positive regulation of cell migration
F	0030424	cellular_component	axon
F	0030425	cellular_component	dendrite
F	0031267	molecular_function	small GTPase binding
F	0031435	molecular_function	mitogen-activated protein kinase kinase kinase binding
F	0032402	biological_process	melanosome transport
F	0032839	cellular_component	dendrite cytoplasm
F	0032872	biological_process	regulation of stress-activated MAPK cascade
F	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
F	0034111	biological_process	negative regulation of homotypic cell-cell adhesion
F	0036064	cellular_component	ciliary basal body
F	0038127	biological_process	ERBB signaling pathway
F	0038133	biological_process	ERBB2-ERBB3 signaling pathway
F	0042552	biological_process	myelination
F	0043204	cellular_component	perikaryon
F	0043410	biological_process	positive regulation of MAPK cascade
F	0043539	molecular_function	protein serine/threonine kinase activator activity
F	0044877	molecular_function	protein-containing complex binding
F	0045893	biological_process	positive regulation of DNA-templated transcription
F	0045933	biological_process	positive regulation of muscle contraction
F	0047496	biological_process	vesicle transport along microtubule
F	0048009	biological_process	insulin-like growth factor receptor signaling pathway
F	0048313	biological_process	Golgi inheritance
F	0048471	cellular_component	perinuclear region of cytoplasm
F	0048678	biological_process	response to axon injury
F	0048812	biological_process	neuron projection morphogenesis
F	0051384	biological_process	response to glucocorticoid
F	0070328	biological_process	triglyceride homeostasis
F	0070371	biological_process	ERK1 and ERK2 cascade
F	0070372	biological_process	regulation of ERK1 and ERK2 cascade
F	0070374	biological_process	positive regulation of ERK1 and ERK2 cascade
F	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
F	0090170	biological_process	regulation of Golgi inheritance
F	0090398	biological_process	cellular senescence
F	0097110	molecular_function	scaffold protein binding
F	0098696	biological_process	regulation of neurotransmitter receptor localization to postsynaptic specialization membrane
F	0098978	cellular_component	glutamatergic synapse
F	0106310	molecular_function	protein serine kinase activity
F	1903298	biological_process	negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
F	2000641	biological_process	regulation of early endosome to late endosome transport
F	2001171	biological_process	positive regulation of ATP biosynthetic process

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGAGNGGVVFkVshkpsglv..........MARK

Chain	Residue	Details
E	LEU74-LYS97
A	ILE355-LYS375

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. ImHrDVKpsNILV

Chain	Residue	Details
E	ILE186-VAL198
A	ILE464-LEU476

---

site_id	PS00479
Number of Residues	46
Details	ZF_DAG_PE_1 Zinc finger phorbol-ester/DAG-type signature. HnFarktflklaf.CdiCqkfLlngfr.....CqtCgykfHehCstkvptm..C

Chain	Residue	Details
A	HIS139-CYS184

---

site_id	PS00796
Number of Residues	11
Details	1433_1 14-3-3 proteins signature 1. RNLLSVAYKNV

Chain	Residue	Details
C	ARG44-VAL54

---

site_id	PS00797
Number of Residues	20
Details	1433_2 14-3-3 proteins signature 2. YKDSTLIMQLLRDNLTLWTS

Chain	Residue	Details
C	TYR217-SER236

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Proton acceptor"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	18
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Modified residue: {"description":"Phosphotyrosine; by SRC","evidences":[{"source":"PubMed","id":"16892053","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"16093354","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"11447113","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"11447113","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Modified residue: {"description":"Symmetric dimethylarginine; by PRMT5","evidences":[{"source":"PubMed","id":"21917714","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"10801873","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16892053","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21917714","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8349614","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10027","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19161339","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3EQH","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	20
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15543157","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17880056","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18951019","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19019675","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19706763","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21310613","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19161339","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3EQF","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine; by BRAF and RAF1","evidences":[{"source":"PubMed","id":"10409742","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20956560","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"29433126","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8131746","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine; by BRAF and RAF1","evidences":[{"source":"PubMed","id":"20956560","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"29433126","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8131746","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---