8CAU

human alpha7 nicotinic receptor in complex with the C4 nanobody and nicotine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E (A, B, C, D, E)	Neuronal acetylcholine receptor subunit alpha-7	polymer	492	55367.7	5	UniProt (P36544) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)
2	F, G, H, I, J (F, G, H, I, J)	Nanobody C4	polymer	147	15826.4	5		Vicugna pacos
3	K, L, M, N, O (P, Q, R, S, T)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	5	In PDB GlyTouCan (G42666HT)
4	BA, CA, P, Q, S... (E, A, B, C, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	10	Chemie (NAG)
5	AA, DA, R, U, X (D, E, A, B, C)	(S)-3-(1-METHYLPYRROLIDIN-2-YL)PYRIDINE	non-polymer		162.2	5	Chemie (NCT)

Sequence modifications

A, B, C, D, E: 1 - 479 (UniProt: P36544)

PDB	External Database	Details
Ser 480	-	expression tag
Ala 481	-	expression tag
Gly 482	-	expression tag
Leu 483	-	expression tag
Thr 484	-	expression tag
Glu 485	-	expression tag
Thr 486	-	expression tag
Ser 487	-	expression tag
Gln 488	-	expression tag
Val 489	-	expression tag
Ala 490	-	expression tag
Pro 491	-	expression tag
Ala 492	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	355970.4
Branched	Number of molecules	5
	Total formula weight	2122.0
Non-Polymers*	Number of molecules	15
	Total formula weight	3023.2
All*	Total formula weight	361115.6

*Water molecules are not included.