8C82

Cryo-EM structure of the yeast SPT-Orm1-Dimer complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Protein ORM1	polymer	222	25221.7	2	UniProt (P53224) Pfam (PF04061) In PDB	Saccharomyces cerevisiae (baker's yeast)
2	B, F	Serine palmitoyltransferase 1	polymer	558	62266.8	2	UniProt (P25045) Pfam (PF00155) In PDB	Saccharomyces cerevisiae (baker's yeast)	SPT 1,SPT1,Long chain base biosynthesis protein 1
3	C, G	Serine palmitoyltransferase 2	polymer	561	63189.7	2	UniProt (P40970) Pfam (PF00155) In PDB	Saccharomyces cerevisiae (baker's yeast)	SPT 2,Long chain base biosynthesis protein 2
4	D, H	Serine palmitoyltransferase-regulating protein TSC3	polymer	80	9590.2	2	UniProt (Q3E790) In PDB	Saccharomyces cerevisiae (baker's yeast)	Temperature-sensitive CSG2-mutant suppressor protein 3
5	A, E	N-[(2S,3S,4R)-1,3,4-trihydroxyoctadecan-2-yl]hexacosanamide	non-polymer		696.2	2	Chemie (Z8A)
6	C, G	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
7	C, E	2-{[(4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25R)-spirost-5-en-3-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside	non-polymer		1165.3	2	Chemie (Q7G)

Sequence modifications

A, E: 1 - 222 (UniProt: P53224)

PDB	External Database	Details
Ala 51	Ser 51	engineered mutation
Ala 52	Ser 52	engineered mutation
Ala 53	Ser 53	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	320536.8
Non-Polymers*	Number of molecules	6
	Total formula weight	4217.3
All*	Total formula weight	324754.1

*Water molecules are not included.