8C82
Cryo-EM structure of the yeast SPT-Orm1-Dimer complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, E) | Protein ORM1 | polymer | 222 | 25221.7 | 2 | UniProt (P53224) Pfam (PF04061) | Saccharomyces cerevisiae (baker's yeast) | |
| 2 | B, F (B, F) | Serine palmitoyltransferase 1 | polymer | 558 | 62266.8 | 2 | UniProt (P25045) Pfam (PF00155) | Saccharomyces cerevisiae (baker's yeast) | SPT 1,SPT1,Long chain base biosynthesis protein 1 |
| 3 | C, G (C, G) | Serine palmitoyltransferase 2 | polymer | 561 | 63189.7 | 2 | UniProt (P40970) Pfam (PF00155) | Saccharomyces cerevisiae (baker's yeast) | SPT 2,Long chain base biosynthesis protein 2 |
| 4 | D, H (D, H) | Serine palmitoyltransferase-regulating protein TSC3 | polymer | 80 | 9590.2 | 2 | UniProt (Q3E790) | Saccharomyces cerevisiae (baker's yeast) | Temperature-sensitive CSG2-mutant suppressor protein 3 |
| 5 | I, L (A, E) | N-[(2S,3S,4R)-1,3,4-trihydroxyoctadecan-2-yl]hexacosanamide | non-polymer | 696.2 | 2 | Chemie (Z8A) | |||
| 6 | J, N (C, G) | PYRIDOXAL-5'-PHOSPHATE | non-polymer | 247.1 | 2 | Chemie (PLP) | |||
| 7 | K, M (C, E) | 2-{[(4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25R)-spirost-5-en-3-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside | non-polymer | 1165.3 | 2 | Chemie (Q7G) |
Sequence modifications
A, E: 1 - 222 (UniProt: P53224)
| PDB | External Database | Details |
|---|---|---|
| Ala 51 | Ser 51 | engineered mutation |
| Ala 52 | Ser 52 | engineered mutation |
| Ala 53 | Ser 53 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 320536.8 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 4217.3 | |
| All* | Total formula weight | 324754.1 |
