Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8C82

Cryo-EM structure of the yeast SPT-Orm1-Dimer complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003674	molecular_function	molecular_function
A	0005515	molecular_function	protein binding
A	0005783	cellular_component	endoplasmic reticulum
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0006672	biological_process	ceramide metabolic process
A	0006986	biological_process	response to unfolded protein
A	0016020	cellular_component	membrane
A	0017059	cellular_component	serine palmitoyltransferase complex
A	0030148	biological_process	sphingolipid biosynthetic process
A	0090155	biological_process	negative regulation of sphingolipid biosynthetic process
A	0090156	biological_process	intracellular sphingolipid homeostasis
B	0004758	molecular_function	serine C-palmitoyltransferase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005783	cellular_component	endoplasmic reticulum
B	0005789	cellular_component	endoplasmic reticulum membrane
B	0006665	biological_process	sphingolipid metabolic process
B	0009058	biological_process	biosynthetic process
B	0016020	cellular_component	membrane
B	0016746	molecular_function	acyltransferase activity
B	0017059	cellular_component	serine palmitoyltransferase complex
B	0030148	biological_process	sphingolipid biosynthetic process
B	0030170	molecular_function	pyridoxal phosphate binding
B	0046512	biological_process	sphingosine biosynthetic process
B	0046513	biological_process	ceramide biosynthetic process
B	0090156	biological_process	intracellular sphingolipid homeostasis
C	0004758	molecular_function	serine C-palmitoyltransferase activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005783	cellular_component	endoplasmic reticulum
C	0006665	biological_process	sphingolipid metabolic process
C	0009058	biological_process	biosynthetic process
C	0016020	cellular_component	membrane
C	0016740	molecular_function	transferase activity
C	0016746	molecular_function	acyltransferase activity
C	0017059	cellular_component	serine palmitoyltransferase complex
C	0030148	biological_process	sphingolipid biosynthetic process
C	0030170	molecular_function	pyridoxal phosphate binding
C	0046512	biological_process	sphingosine biosynthetic process
C	0046513	biological_process	ceramide biosynthetic process
C	0090156	biological_process	intracellular sphingolipid homeostasis
D	0005783	cellular_component	endoplasmic reticulum
D	0005789	cellular_component	endoplasmic reticulum membrane
D	0006666	biological_process	3-keto-sphinganine metabolic process
D	0008047	molecular_function	enzyme activator activity
D	0016020	cellular_component	membrane
D	0017059	cellular_component	serine palmitoyltransferase complex
D	0090154	biological_process	positive regulation of sphingolipid biosynthetic process
D	0090156	biological_process	intracellular sphingolipid homeostasis
E	0003674	molecular_function	molecular_function
E	0005515	molecular_function	protein binding
E	0005783	cellular_component	endoplasmic reticulum
E	0005789	cellular_component	endoplasmic reticulum membrane
E	0006672	biological_process	ceramide metabolic process
E	0006986	biological_process	response to unfolded protein
E	0016020	cellular_component	membrane
E	0017059	cellular_component	serine palmitoyltransferase complex
E	0030148	biological_process	sphingolipid biosynthetic process
E	0090155	biological_process	negative regulation of sphingolipid biosynthetic process
E	0090156	biological_process	intracellular sphingolipid homeostasis
F	0004758	molecular_function	serine C-palmitoyltransferase activity
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005783	cellular_component	endoplasmic reticulum
F	0005789	cellular_component	endoplasmic reticulum membrane
F	0006665	biological_process	sphingolipid metabolic process
F	0009058	biological_process	biosynthetic process
F	0016020	cellular_component	membrane
F	0016746	molecular_function	acyltransferase activity
F	0017059	cellular_component	serine palmitoyltransferase complex
F	0030148	biological_process	sphingolipid biosynthetic process
F	0030170	molecular_function	pyridoxal phosphate binding
F	0046512	biological_process	sphingosine biosynthetic process
F	0046513	biological_process	ceramide biosynthetic process
F	0090156	biological_process	intracellular sphingolipid homeostasis
G	0004758	molecular_function	serine C-palmitoyltransferase activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005783	cellular_component	endoplasmic reticulum
G	0006665	biological_process	sphingolipid metabolic process
G	0009058	biological_process	biosynthetic process
G	0016020	cellular_component	membrane
G	0016740	molecular_function	transferase activity
G	0016746	molecular_function	acyltransferase activity
G	0017059	cellular_component	serine palmitoyltransferase complex
G	0030148	biological_process	sphingolipid biosynthetic process
G	0030170	molecular_function	pyridoxal phosphate binding
G	0046512	biological_process	sphingosine biosynthetic process
G	0046513	biological_process	ceramide biosynthetic process
G	0090156	biological_process	intracellular sphingolipid homeostasis
H	0005783	cellular_component	endoplasmic reticulum
H	0005789	cellular_component	endoplasmic reticulum membrane
H	0006666	biological_process	3-keto-sphinganine metabolic process
H	0008047	molecular_function	enzyme activator activity
H	0016020	cellular_component	membrane
H	0017059	cellular_component	serine palmitoyltransferase complex
H	0090154	biological_process	positive regulation of sphingolipid biosynthetic process
H	0090156	biological_process	intracellular sphingolipid homeostasis

Functional Information from PROSITE/UniProt

site_id	PS00599
Number of Residues	10
Details	AA_TRANSFER_CLASS_2 Aminotransferases class-II pyridoxal-phosphate attachment site. TFTKSFGAAG

Chain	Residue	Details
C	THR363-GLY372

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	40
Details	TRANSMEM: Helical => ECO:0000255

Chain	Residue	Details
D	PHE54-ILE74
H	PHE54-ILE74
G	PRO57-VAL77
G	LEU443-PRO463
E	LYS131-LYS162
E	HIS206-SER222

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: N6-(pyridoxal phosphate)lysine => ECO:0000250

Chain	Residue	Details
C	LYS366
G	LYS366
B	ALA425-LYS457
F	HIS50-GLN84
F	ASP342-MET371
F	ALA425-LYS457
E	PHE163-LEU183
E	LEU185-THR205

site_id	SWS_FT_FI3
Number of Residues	712
Details	TOPO_DOM: Cytoplasmic => ECO:0000269\|PubMed:15485854

Chain	Residue	Details
B	LYS85-MET341
B	PHE458-LYS558
F	LYS85-MET341
F	PHE458-LYS558

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:17330950

Chain	Residue	Details
B	THR121
F	THR121

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:17330950, ECO:0007744\|PubMed:19779198

Chain	Residue	Details
A	SER32
E	SER32

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:19779198

Chain	Residue	Details
A	SER56
E	SER56

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)